L. 和 Rosc. 提取物对埃塞俄比亚哈迪亚地区丝虫病传播媒介的杀幼虫活性。
Larvicidal Activities of L. and Rosc. Extracts against Filariasis Vectors in Hadiya Zone, Ethiopia.
机构信息
Department of Biology, Wachemo University, P.O. Box 667, Hossana, Ethiopia.
Department of Biotechnology, Wachemo University, PO Box 667, Hossana, Ethiopia.
出版信息
Biomed Res Int. 2023 May 31;2023:6636837. doi: 10.1155/2023/6636837. eCollection 2023.
Mosquitoes present an immense threat to millions of people worldwide and act as vectors for filariasis disease. The objective of the study was to determine the effect of and extracts against filariasis vectors. The larvae were collected from the breeding site by using standard procedures for identification and larvicidal activities. Twenty grams (20 g) from each ( and ) were extracted separately by aqueous, ethanol, and methanol solvents. The phytochemical analysis was determined in the crude sample by using standard methods. Then, larvicidal effects were determined by introducing 10 larvae of the vectors to the concentrations of 250 ppm, 500 ppm, and 750 ppm of the crude sample, and data were subjected to probit analysis to determine the LC and Chi-squared test to check the significance of the mortality by R software. , s.l., , , and were the filariasis vectors identified during the study period. The presence of phytochemical tests such as anthraquinones, flavonoids, glycosides, phenol, saponin, steroids, tannin, and terpenes was obtained. The larvicidal effects of the selected plant extracts ranged from 0%-100%. The lowest LC (53 ppm) was observed for methanol test extract against . Ethanol extracts of have a significant effect on ( = 7.5, = 0.02352) and ( = 10.833, = 0.0.0044), whereas aqueous extracts have a significant effect only on s.l. ( = 7.0807, = 0.029. Ethanol extracts of have a significant effect only on the mortality of ( = 7.0807, = 0.029), but methanol and aqueous extracts have no significant effect against filariasis vectors. In conclusion, have a high toxic effect than extract against filariasis vectors in all type of solvents. So using those plant extracts is the best to reduce the risk of the synthetic chemical on nontarget organisms and the environment, in addition to the control of mosquito-borne diseases, but further studies will be conducted to evaluate the toxicity at different stages of the vectors.
蚊子对全球数百万人构成巨大威胁，并充当丝虫病的传播媒介。本研究的目的是确定 和 提取物对丝虫病媒介的影响。幼虫通过标准程序从繁殖地收集，并进行鉴定和杀幼虫活性测试。从每个（ 和 ）中提取 20 克（20 g），分别用含水、乙醇和甲醇溶剂提取。通过标准方法确定粗提物中的植物化学成分。然后，将 10 只蚊幼虫引入浓度为 250 ppm、500 ppm 和 750 ppm 的粗提物中，进行杀幼虫效果测试，并通过 R 软件的概率分析确定 LC 值和卡方检验来检查死亡率的显著性。在研究期间，鉴定出 的 s.l.、 、 、 和 为丝虫病媒介。获得了植物化学测试结果，如蒽醌、类黄酮、糖苷、苯酚、皂苷、类固醇、单宁和萜烯。选定植物提取物的杀幼虫效果为 0%-100%。 甲醇测试提取物对 的最低 LC（53 ppm）。 乙醇提取物对 和 （ = 7.5， = 0.02352）和 （ = 10.833， = 0.0.0044）有显著影响，而含水提取物仅对 s.l.（ = 7.0807， = 0.029）有显著影响。 乙醇提取物仅对 的死亡率有显著影响（ = 7.0807， = 0.029），但甲醇和含水提取物对丝虫病媒介没有显著影响。总之， 和 提取物在所有溶剂类型中对丝虫病媒介都有较高的毒性作用。因此，使用这些植物提取物是减少合成化学品对非目标生物和环境的风险以及控制蚊媒疾病的最佳方法，但还需要进行进一步的研究来评估不同阶段媒介的毒性。
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