Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Menoufia University, Shebein Alkom, Egypt.
Department of Biochemistry, Faculty of Medicine, UQU, Makkah, Saudi Arabia.
Mol Biol Rep. 2023 Jul;50(7):6075-6085. doi: 10.1007/s11033-023-08569-0. Epub 2023 Jun 9.
LncRNAs may play a role in either suppressing or exacerbating diabetes-associated vascular complications.
This study aimed to assess MEG3 and H19 expression levels in T2DM and pre-diabetes and their roles in diabetes-related microvascular complications.
(RT-PCR) analysis of the MEG3 and H19 plasma levels was carried out in 180 participants of T2DM, pre-diabetes, and control.
The expression level of lncRNA H19 was significantly down-regulated and lncRNA MEG3 up-regulated in T2DM compared to pre-diabetes and control, also for pre-diabetes versus control. The (ROC) analysis of MEG3 and H19 relative expression levels showed that MEG3 has better sensitivity for distinguishing T2DM from pre-diabetes and control groups.In comparison, H19 offered superior sensitivity to distinguish pre-diabetic from controls. Additionally, H19 was reported as an independent risk factor for T2DM by multivariate analysis. Low expression of H19 and over-expressed MEG3 were significantly associated with retinopathy, nephropathy, and elevated renal indicators (urea, creatinine, and UACR.
Our results implicated the potential diagnostic and predictive roles of lncRNA MEG3 and H19 for T2DM and related microvascular complications. Additionally, H19 may serve as a potential biomarker for pre-diabetes prediction.
lncRNAs 可能在抑制或加剧糖尿病相关血管并发症方面发挥作用。
本研究旨在评估 T2DM 和糖尿病前期患者中 MEG3 和 H19 的表达水平及其在糖尿病相关微血管并发症中的作用。
对 180 例 T2DM、糖尿病前期和对照组患者的 MEG3 和 H19 血浆水平进行（RT-PCR）分析。
与糖尿病前期和对照组相比，T2DM 患者的 lncRNA H19 表达水平显著下调，MEG3 表达水平上调，糖尿病前期患者与对照组相比也存在这种情况。MEG3 和 H19 相对表达水平的（ROC）分析表明，MEG3 对区分 T2DM 与糖尿病前期和对照组具有更好的敏感性。相比之下，H19 对区分糖尿病前期与对照组具有更高的敏感性。此外，多变量分析显示 H19 是 T2DM 的独立危险因素。H19 低表达和 MEG3 过表达与视网膜病变、肾病和升高的肾脏指标（尿素、肌酐和 UACR）显著相关。
我们的结果表明 lncRNA MEG3 和 H19 对 T2DM 及相关微血管并发症具有潜在的诊断和预测作用。此外，H19 可能是预测糖尿病前期的潜在生物标志物。
