Alfaifi Mohammed, Verma Amit Kumar, Alshahrani Mohammad Yahya, Joshi Prakash C, Alkhathami Ali G, Ahmad Irfan, Hakami Abdulrahim Refdan, Beg Mirza Masroor Ali
Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Khalid University, Abha, Saudi Arabia.
Department of Zoology and Environmental Sciences, GKV, Haridwar, India.
Diabetes Metab Syndr Obes. 2020 Oct 14;13:3727-3737. doi: 10.2147/DMSO.S273586. eCollection 2020.
Type 2 diabetes mellitus [T2DM] has been one of the common diseases and is characterized by increased blood glucose levels and suggested that cell-free non-coding RNAs and microRNAs (miRNAs) have been demonstrated to serve as important diagnostic/prognostic biomarkers in diabetes.
MATERIALS/METHODS: The present study included clinically confirmed newly diagnosed 200 cases of T2DM and 200 healthy subjects, and all the parameters were taken care in diagnosis. Blood samples collected in plain vials were used for cell-free total RNA extraction and after that 100ng of total RNA was used to synthesize the cDNA for cell-free lncRNA H19, miRNA-29a, and miRNA-29b expression using quantitative real-time PCR method. Serum Biochemical parameters were analyzed after collection of the sample to observe the changes among T2DM cases and healthy controls.
It was observed that type 2 diabetic patients had decreased [0.59 fold] lncRNA H19 expression while increased miRNA-29a [5.62 fold] and miRNA-29b [5.58 fold] expression. Decreased expression of lncRNA H19 was observed to be associated with gender [p=0.004], hypertension [p<0.0001], weight loss [p=0.02] and fatigue [p=0.02]. Increased miRNA29a expression was linked with hypertension [p<0.0001], alcoholism [p=0.04], and smoking [p<0.0001] as well as miRNA-29b expression was associated with hypertension [p=0.0001], weight loss [p=0.002], smoking [p=0.0002], alcoholism [p<0.0001]. Low [≤1 fold] and high [>1 fold] expression of lncRNA H19 expression was linked with miRNA-29a [p=0.005] and miRNA-29b [p<0.0001] expression. lncRNA H19 expression showed negative correlation with miRNA-29a expression [r= -27, p<0.0001] and miRNA-29b [r= -47, p<0.0001].
The present study concluded that lower lncRNA H19 expression, and increased miRNA-29b, a miRNA-29b expression associated with the severity of T2DM patients. Decreased lncRNA H19 expression, and increased miRNA-29b, miRNA-29b expression observed to be interrelated with clinicopathological findings of T2DM patients could involve in pathogenesis disease.
2型糖尿病（T2DM）一直是常见疾病之一，其特征为血糖水平升高，且无细胞非编码RNA和微小RNA（miRNA）已被证明可作为糖尿病重要的诊断/预后生物标志物。
材料/方法：本研究纳入200例临床确诊的新诊断T2DM患者和200名健康受试者，所有参数在诊断时均予以关注。用普通采血管采集的血样用于无细胞总RNA提取，之后用100ng总RNA通过定量实时PCR法合成用于检测无细胞lncRNA H19、miRNA - 29a和miRNA - 29b表达的cDNA。采集样本后分析血清生化参数，以观察T2DM患者与健康对照之间的变化。
观察到2型糖尿病患者lncRNA H19表达降低[0.59倍]，而miRNA - 29a[5.62倍]和miRNA - 29b[5.58倍]表达升高。观察到lncRNA H19表达降低与性别[p = 0.004]、高血压[p < 0.0001]、体重减轻[p = 0.02]和疲劳[p = 0.02]有关。miRNA29a表达升高与高血压[p < 0.0001]、酗酒[p = 0.04]和吸烟[p < 0.0001]有关，miRNA - 29b表达与高血压[p = 0.0001]、体重减轻[p = 0.002]、吸烟[p = 0.0002]、酗酒[p < 0.0001]有关。lncRNA H19表达低[≤1倍]和高[>1倍]与miRNA - 29a[p = 0.005]和miRNA - 29b[p < 0.0001]表达有关。lncRNA H19表达与miRNA - 29a表达呈负相关[r = -27，p < 0.0001]，与miRNA - 29b[r = -47，p < 0.0001]呈负相关。
本研究得出结论，lncRNA H19表达降低以及miRNA - 29b升高，miRNA - 29b表达与T2DM患者的严重程度相关。观察到lncRNA H19表达降低以及miRNA - 29b、miRNA - 29b表达升高与T2DM患者的临床病理结果相关，可能参与疾病的发病机制。
