Ochiai A, Tahara E
Gan To Kagaku Ryoho. 1986 Apr;13(4 Pt 2):1416-22.
A new mouse monoclonal antibody 9A3 (IgGl,K) raised against human poorly differentiated gastric adenocarcinoma cell line TMK-1 was produced. Immunohistochemically, 9A3 antibody reacted strongly with gastric carcinoma, colon carcinoma, pancreas carcinoma, lung carcinoma, breast carcinoma and cervical carcinoma of the uterus. This antibody did not react with various normal tissues from the whole body with the exception of neutrophils and macrophages. In fetal tissues, 9A3 antibody reacted with esophageal mucosa and colon epithelium, but did not react with purified carcinoembryonic antigen (CEA). The molecular weight of the antigen extracted with NP-40 from TMK-1 cells was estimated to be about 46,000 daltons by SDS-PAGE. The 9A3 antigen was also detected in conditioned tissue culture medium of the TMK-1 cell line and sera of gastric cancer patients and tumor imaging could be performed in xenotransplantable human gastric carcinoma in nude mice. In summary, 9A3 antibody may serve as a new marker for detection of cancer by immunohistochemical, cytological techniques and serologically in the sera of cancer patients.
制备了一种新的针对人低分化胃腺癌细胞系TMK-1产生的小鼠单克隆抗体9A3（IgG1，K）。免疫组织化学检测显示，9A3抗体与胃癌、结肠癌、胰腺癌、肺癌、乳腺癌和子宫颈癌有强烈反应。除中性粒细胞和巨噬细胞外，该抗体与全身各种正常组织均无反应。在胎儿组织中，9A3抗体与食管黏膜和结肠上皮反应，但与纯化的癌胚抗原（CEA）无反应。通过SDS-PAGE估计，用NP-40从TMK-1细胞中提取的抗原分子量约为46,000道尔顿。在TMK-1细胞系的条件组织培养基和胃癌患者血清中也检测到了9A3抗原，并且可以在裸鼠体内的可异种移植人胃癌中进行肿瘤成像。总之，9A3抗体可作为一种新的标志物，通过免疫组织化学、细胞学技术以及癌症患者血清学检测来检测癌症。
