Key Laboratory for Industrial Microbiology of Henan Province, School of Biological and Chemical Engineering, Nanyang Institute of Technology, Nanyang 473000, PR China.
Agricultural Bio-resources Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou City, Fujian Province 350003, PR China.
FEMS Microbiol Lett. 2023 Jan 17;370. doi: 10.1093/femsle/fnad054.
In the present study, the taxonomic position of Bacillus dafuensis and Bacillus massiliigabonensis was evaluated using genome-based comparison. The 16S rRNA gene sequence obtained from the Bacillus dafuensis FJAT-25496T genome showed 99.7% similarity with the type strain of Cytobacillus citreus, while Bacillus massiliigabonensis Marseille-P2639T showed 98.7% similarity with the type species of Cytobacillus solani. The 16S rRNA gene sequence similarity of Bacillus dafuensis FJAT-25496T and Bacillus massiliigabonensis Marseille-P2639T with Cytobacillus members was above the threshold (94.5%) for genus delineation. In phylogenetic (based on 16S rRNA gene sequences) and phylogenomic (based on 71 bacterial single-copy genes) trees, Bacillus dafuensis and Bacillus massiliigabonensis clustered with Cytobacillus members. The 16S rRNA gene sequence, amino acid identity and percentage of conserved proteins analysis indicated Bacillus dafuensis FJAT-25496T and Bacillus massiliigabonensis Marseille-P2639T as a member of the genus Cytobacillus. The digital DNA-DNA hybridization and the average nucleotide identity values of Bacillus dafuensis FJAT-25496T and Bacillus massiliigabonensis Marseille-P2639T with Cytobacillus members was below the cut-off value (70/94%-95%) for species delineation. Based on the results we propose to transfer Bacillus dafuensis and Bacillus massiliigabonensis to the genus Cytobacillus as Cytobacillus dafuensis comb. nov., and Cytobacillus massiliigabonensis comb. nov., respectively.
在本研究中，通过基于基因组的比较评估了大福芽孢杆菌和马塞加博芽孢杆菌的分类地位。从大福芽孢杆菌 FJAT-25496T 基因组中获得的 16S rRNA 基因序列与柠檬酸芽孢杆菌的模式株具有 99.7%的相似性，而马塞加博芽孢杆菌 Marseille-P2639T 与梭状芽孢杆菌的模式种具有 98.7%的相似性。大福芽孢杆菌 FJAT-25496T 和马塞加博芽孢杆菌 Marseille-P2639T 与梭状芽孢杆菌成员的 16S rRNA 基因序列相似性高于属划界的阈值（94.5%）。在系统发育（基于 16S rRNA 基因序列）和系统基因组学（基于 71 个细菌单拷贝基因）树中，大福芽孢杆菌和马塞加博芽孢杆菌与梭状芽孢杆菌成员聚类。16S rRNA 基因序列、氨基酸身份和保守蛋白分析的百分比表明，大福芽孢杆菌 FJAT-25496T 和马塞加博芽孢杆菌 Marseille-P2639T 是梭状芽孢杆菌属的成员。大福芽孢杆菌 FJAT-25496T 和马塞加博芽孢杆菌 Marseille-P2639T 与梭状芽孢杆菌成员的数字 DNA-DNA 杂交和平均核苷酸身份值低于种划界的截止值（70/94%-95%）。基于这些结果，我们建议将大福芽孢杆菌和马塞加博芽孢杆菌转移到梭状芽孢杆菌属中，分别作为大福梭状芽孢杆菌 comb. nov.和马塞加博梭状芽孢杆菌 comb. nov.。
