Bardyn Manon, Martin Agathe, Dögnitz Nora, Abonnenc Mélanie, Dunham Andrew, Yoshida Tatsuro, Prudent Michel
Laboratoire de Recherche sur les Produits Sanguins, Transfusion Interrégionale CRS, Epalinges, Switzerland.
Laboratoire de Préparation Cellulaire et d'Analyses, Transfusion Interrégionale CRS, Epalinges, Switzerland.
Front Physiol. 2020 Dec 23;11:616457. doi: 10.3389/fphys.2020.616457. eCollection 2020.
Unexpectedly wide distribution (<10 to >90%) of hemoglobin oxygen saturation (sO) within red cell concentrates (RCCs) has recently been observed. Causes of such variability are not yet completely explained whereas the roles of oxygen and oxidative lesions during the storage of RCCs are known. The objectives of the present study are to characterize sO distribution in RCCs produced in a Swiss blood center and to investigate the influence of processing and donors' characteristics. The level of sO was measured in 1701 leukocyte-depleted RCCs derived from whole blood donations in both top-bottom (TB; component filtered, SAGM) and top-top (TT; whole blood filtration, PAGGSM) RCCs. The sO value was measured non-invasively through the PVC bag prior to storage by resonance Raman spectroscopy. Gender, age, blood type, hemoglobin level, and living altitude of donors, as well as process method and time-to-process were recorded. Overall, the sO exhibited a wide non-Gaussian distribution with a mean of 51.2 ± 18.5%. Use of top-top kits resulted in a 16% higher sO ( < 0.0001) than with top-bottom ones. Waiting time before processing only had a modest impact, but the blood processing itself reduced the sO by almost 12% ( < 0.0001). sO was also significantly affected by some donors' characteristics. RCCs from men exhibited 25% higher sO ( < 0.0001) than those donated by women. Multivariate analysis revealed that the apparent correlation observed with hemoglobin level and age was actually due to multicollinearity with the sex variable. Finally, we noticed no significant differences across blood type but found that altitude of residence was associated with the sO (i.e., higher in higher living place). These data confirm wide sO distribution in RCCs reported recently. The sO was impacted by the processing and also by donors' characteristics such as the gender and the living altitude, but not by the hemoglobin level, blood group and donor age. This study provides new hints on the factors influencing red blood cells storage lesions, since they are known to be related to O content within the bags, giving clues to better process and to better store RCCs and therefore potentially improve the efficacy of transfusion.
最近观察到红细胞浓缩物（RCC）中血红蛋白氧饱和度（sO）存在意想不到的广泛分布（<10%至>90%）。这种变异性的原因尚未完全阐明，而RCC储存期间氧和氧化损伤的作用是已知的。本研究的目的是描述瑞士一家血液中心生产的RCC中sO的分布特征，并研究加工过程和献血者特征的影响。在1701份源自全血捐献的白细胞去除RCC中测量了sO水平，这些RCC分别采用上下（TB；成分过滤，SAGM）和上顶（TT；全血过滤，PAGGSM）方法制备。在储存前通过共振拉曼光谱法对PVC袋进行无创测量sO值。记录了献血者的性别、年龄、血型、血红蛋白水平和居住海拔，以及加工方法和加工时间。总体而言，sO呈现出广泛的非高斯分布，平均值为51.2±18.5%。使用上顶试剂盒导致sO比上下试剂盒高16%（<0.0001）。加工前的等待时间影响不大，但血液加工本身使sO降低了近12%（<0.0001）。sO也受到一些献血者特征的显著影响。男性捐献的RCC的sO比女性捐献的高25%（<0.0001）。多变量分析显示，观察到的与血红蛋白水平和年龄的明显相关性实际上是由于与性别变量的多重共线性。最后，我们注意到不同血型之间没有显著差异，但发现居住海拔与sO有关（即居住海拔越高，sO越高）。这些数据证实了最近报道的RCC中sO的广泛分布。sO受到加工过程以及献血者特征（如性别和居住海拔）的影响，但不受血红蛋白水平、血型和献血者年龄的影响。这项研究为影响红细胞储存损伤的因素提供了新的线索，因为已知这些损伤与袋内的氧含量有关，为更好地加工和储存RCC提供了线索，从而有可能提高输血效果。
