Osawa T, Ide C, Tohyama K
Arch Histol Jpn. 1986 Mar;49(1):69-81. doi: 10.1679/aohc.49.69.
The purpose of this study was to examine whether the basal laminae of Schwann cells in allografts could survive immunological rejection and serve as a conduit for regenerating nerves, as in the case of autogenic nerve grafts. Allografts of nerves were carried out using sciatic nerves of mice after the grafts had been repetitively frozen to kill their Schwann cells. Two mouse strains, C57BL/6N and C3H/HeN, were used, as they are known to differ in major histocompatibility complex. The mid-portion of the grafted nerve segments was examined by electron microscopy. In addition, the toe pad skin and lumbrical muscles were examined for determining whether regenerating nerves reinnervate sensory end organs and motor endplates. The process of nerve regeneration in the allograft was the same as that seen in the autograft. Cells in the graft disintegrated into cell debris and were phagocytized by macrophages, whereas the basal laminae of Schwann cells were not removed by macrophages, remaining in the form of tubes or scaffolds. Regenerating nerve fibers grew out through such basal lamina scaffolds, keeping in contact with the inner surface. Digital sensory corpuscles and motor endplates of the operated side were well reinnervated. The results indicate that the basal laminae of Schwann cells of the allograft may survive and serve as a conduit for regenerating axons in the same way as in the case of an autograft.
本研究的目的是检验同种异体移植中雪旺细胞的基底层是否能够在免疫排斥反应中存活，并像自体神经移植那样作为神经再生的管道。在将神经移植体反复冷冻以杀死其雪旺细胞后，使用小鼠的坐骨神经进行神经同种异体移植。使用了两种小鼠品系，C57BL/6N和C3H/HeN，因为已知它们在主要组织相容性复合体方面存在差异。通过电子显微镜检查移植神经段的中部。此外，检查趾垫皮肤和蚓状肌，以确定再生神经是否重新支配感觉终器和运动终板。同种异体移植中神经再生的过程与自体移植中所见的相同。移植体中的细胞解体为细胞碎片并被巨噬细胞吞噬，而雪旺细胞的基底层未被巨噬细胞清除，以管或支架的形式保留下来。再生神经纤维通过这种基底层支架生长出来，与内表面保持接触。手术侧的数字感觉小体和运动终板得到了良好的重新支配。结果表明，同种异体移植中雪旺细胞的基底层可能存活，并像自体移植那样作为再生轴突的管道。
