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TCP21通过诱导氧化应激和细胞凋亡发挥抗真菌作用。

Antifungal Action of TCP21 via Induction of Oxidative Stress and Apoptosis.

作者信息

Park Seong-Cheol, Yoon A-Mi, Kim Young-Min, Lee Min-Young, Lee Jung Ro

机构信息

Department of Chemical Engineering, Sunchon National University, Suncheon 57922, Republic of Korea.

LMO Team, National Institute of Ecology (NIE), Seocheon 33657, Republic of Korea.

出版信息

Antioxidants (Basel). 2023 Sep 15;12(9):1767. doi: 10.3390/antiox12091767.

DOI:10.3390/antiox12091767
PMID:37760070
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10525234/
Abstract

The realm of antimicrobial proteins in plants is extensive but remains relatively uncharted. Understanding the mechanisms underlying the action of plant antifungal proteins (AFPs) holds promise for antifungal strategies. This study aimed to bridge this knowledge gap by comprehensively screening species to identify novel AFPs. Using MALDI-TOF analysis, we identified a member of the TEOSINTE BRANCHED1/CYCLOIDEA/PROLIFERATING CELL FACTOR1 (TCP) family of transcription factors as a novel AFP, TCP21 (AtTCP21; accession number NP_196450). Bacterially purified recombinant AtTCP21 inhibited the growth of various pathogenic fungal cells. AtTCP21 was more potent than melittin, a well-known AFP, in combating . Growth inhibition assays against various fungal pathogens and yeasts confirmed the pH-dependent antimicrobial activity of AtTCP21. Without inducing any membrane alterations, AtTCP21 penetrates the fungal cell wall and membrane, where it instigates a repressive milieu for fungal cell growth by generating intracellular reactive oxygen species and mitochondrial superoxides; resulting in morphological changes and apoptosis. Our findings demonstrate the redox-regulating effects of AtTCP21 and point to its potential as an antimicrobial agent.

摘要

植物中抗菌蛋白的领域广泛，但仍相对未被充分探索。了解植物抗真菌蛋白（AFP）作用的潜在机制为抗真菌策略带来了希望。本研究旨在通过全面筛选物种以鉴定新型AFP来填补这一知识空白。使用基质辅助激光解吸电离飞行时间（MALDI-TOF）分析，我们鉴定出一种转录因子TEOSINTE BRANCHED1/CYCLOIDEA/PROLIFERATING CELL FACTOR1（TCP）家族的成员作为新型AFP，即TCP21（拟南芥TCP21；登录号NP_196450）。细菌纯化的重组AtTCP21抑制了各种致病真菌细胞的生长。在对抗[此处原文缺失相关内容]方面，AtTCP21比著名的AFP蜂毒肽更有效。针对各种真菌病原体和酵母的生长抑制试验证实了AtTCP21的pH依赖性抗菌活性。AtTCP21在不引起任何膜改变的情况下穿透真菌细胞壁和细胞膜，在其中通过产生细胞内活性氧和线粒体超氧化物为真菌细胞生长营造抑制环境；导致形态变化和细胞凋亡。我们的研究结果证明了AtTCP21的氧化还原调节作用，并指出了其作为抗菌剂的潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82c1/10525234/9c71e84ae78e/antioxidants-12-01767-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82c1/10525234/d8ab1a2270b2/antioxidants-12-01767-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82c1/10525234/751990ccafae/antioxidants-12-01767-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82c1/10525234/391788171fb7/antioxidants-12-01767-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82c1/10525234/873ea7fafdfe/antioxidants-12-01767-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82c1/10525234/658ff160fff9/antioxidants-12-01767-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82c1/10525234/e0e12f6621b2/antioxidants-12-01767-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82c1/10525234/6348b639f571/antioxidants-12-01767-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82c1/10525234/16ec1aca3d19/antioxidants-12-01767-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82c1/10525234/2f07362fc2c8/antioxidants-12-01767-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82c1/10525234/9c71e84ae78e/antioxidants-12-01767-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82c1/10525234/d8ab1a2270b2/antioxidants-12-01767-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82c1/10525234/751990ccafae/antioxidants-12-01767-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82c1/10525234/391788171fb7/antioxidants-12-01767-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82c1/10525234/873ea7fafdfe/antioxidants-12-01767-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82c1/10525234/658ff160fff9/antioxidants-12-01767-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82c1/10525234/e0e12f6621b2/antioxidants-12-01767-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82c1/10525234/6348b639f571/antioxidants-12-01767-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82c1/10525234/16ec1aca3d19/antioxidants-12-01767-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82c1/10525234/2f07362fc2c8/antioxidants-12-01767-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82c1/10525234/9c71e84ae78e/antioxidants-12-01767-g010.jpg

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