Zhang Luhong, Ai Yang, Chen Yunzhu, Li Changzhu, Li Peiwang, Chen Jingzhen, Jiang Lijuan, Pan Yuhong, Sun An, Yang Yan, Liu Qiang
College of Life Science and Technology, Central South University of Forestry and Technology, Changsha 410004, China.
State Key Laboratory of Utilization of Woody Oil Resource, Hunan Academy of Forestry, Changsha 410004, China.
Plants (Basel). 2023 Jun 3;12(11):2209. doi: 10.3390/plants12112209.
fruits are extensively grown worldwide, with a large harvest, and its major medicinal ingredients are geniposide and crocins. Research on their accumulation and biosynthsis-related enzymes is rare. In this study, the accumulation of geniposide and crocin of fruits at different developmental stages were clarified by HPLC. The highest cumulative amount of geniposide was 2.035% during the unripe-fruit period, and the highest content of crocin was 1.098% during the mature-fruit period. Furthermore, transcriptome sequencing was performed. A total of 50 unigenes encoding 4 key enzymes related in geniposide biosynthsis pathways were screened, and 41 unigenes encoding 7 key enzymes in the pathways of crocin were elucidated. It was found that the expression levels of differentially expressed genes of DN67890-encoding , which is highly related to geniposide biosynthesis, and DN81253-encoding , DN79477-encoding , and DN84975-encoding , which are highly related to crocin biosynthesis, were consistent with the accumulation of geniposide and crocin content, respectively. The qRT-PCR results showed that the trends of relative expression were consistent with transcribed genes. This study provides insights for understanding the geniposide and crocin accumulation and biosynthsis during fruit development in .
果实广泛种植于世界各地，产量颇丰，其主要药用成分是栀子苷和西红花苷。关于它们的积累及生物合成相关酶的研究很少。在本研究中，通过高效液相色谱法（HPLC）明确了不同发育阶段果实中栀子苷和西红花苷的积累情况。栀子苷的最高累积量在未成熟果期为2.035%，西红花苷的最高含量在成熟果期为1.098%。此外，还进行了转录组测序。共筛选出50个编码与栀子苷生物合成途径相关的4种关键酶的单基因，阐明了41个编码西红花苷途径中7种关键酶的单基因。发现与栀子苷生物合成高度相关的编码DN67890的差异表达基因，以及与西红花苷生物合成高度相关的编码DN81253、编码DN79477和编码DN84975的差异表达基因的表达水平，分别与栀子苷和西红花苷含量的积累一致。定量逆转录聚合酶链反应（qRT-PCR）结果表明，相对表达趋势与转录基因一致。本研究为了解果实发育过程中栀子苷和西红花苷的积累及生物合成提供了见解。
