Abdellatif Ahmed A H, Mohammed Ahmed M, Saleem Imran, Alsharidah Mansour, Al Rugaie Osamah, Ahmed Fatma, Osman Shaaban K
Department of Pharmaceutics, College of Pharmacy, Qassim University, Buraydah 51452, Saudi Arabia.
Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, Al-Azhar University, Assiut 71524, Egypt.
Pharmaceutics. 2022 Mar 17;14(3):661. doi: 10.3390/pharmaceutics14030661.
The treatment of breast cancer requires long chemotherapy management, which is accompanied by severe side effects. Localized delivery of anticancer drugs helps to increase the drug concentration at the site of action and overcome such a problem. In the present study, chitosan hydrogel was prepared for local delivery of 5-Fluorouracil. The in vitro release behavior was investigated and the anticancer activity was evaluated against MCF-7 cells using MTT assay. The in vivo studies were investigated via intra-tumoral injection of a 5-FU loaded hydrogel into breast cancer of female rats. The results indicated that the modified hydrogel has excellent physicochemical properties with a sustained in vitro release profile matching a zero-order kinetic for one month. In addition, the hydrogel showed superior inhibition of cell viability compared with the untreated control group. Moreover, the in vivo studies resulted in antitumor activity with minor side effects. The tumor volume and level of tumor markers in blood were inhibited significantly by applying the hydrogel compared with the untreated control group. In conclusion, the designed injectable hydrogels are potential drug delivery systems for the treatment of breast cancer with a controlled drug release profile, which could be suitable for decreasing the side effects of chemotherapy agents.
乳腺癌的治疗需要长期的化疗管理，这会伴随着严重的副作用。抗癌药物的局部递送有助于提高作用部位的药物浓度并克服此类问题。在本研究中，制备了壳聚糖水凝胶用于5-氟尿嘧啶的局部递送。研究了其体外释放行为，并使用MTT法评估了对MCF-7细胞的抗癌活性。通过向雌性大鼠乳腺癌瘤内注射负载5-氟尿嘧啶的水凝胶进行体内研究。结果表明，改性水凝胶具有优异的物理化学性质，体外释放曲线持续，符合一级动力学，持续一个月。此外，与未处理的对照组相比，水凝胶对细胞活力的抑制作用更强。此外，体内研究显示出抗肿瘤活性且副作用较小。与未处理的对照组相比，应用水凝胶可显著抑制肿瘤体积和血液中肿瘤标志物的水平。总之，所设计的可注射水凝胶是用于治疗乳腺癌的潜在药物递送系统，具有可控的药物释放曲线，可能适合于减少化疗药物的副作用。
