Szustakiewicz Konrad, Włodarczyk Marcin, Gazińska Małgorzata, Rudnicka Karolina, Płociński Przemysław, Szymczyk-Ziółkowska Patrycja, Ziółkowski Grzegorz, Biernat Monika, Sieja Katarzyna, Grzymajło Michał, Jóźwiak Piotr, Michlewska Sylwia, Trochimczuk Andrzej W
Department of Polymer Engineering and Technology, Faculty of Chemistry, Wrocław University of Science and Technology (WUST), Wyb. Wyspiańskiego 27, 50-370 Wrocław, Poland.
Department of Immunology and Infectious Biology, Faculty of Biology and Environmental Protection, University of Łódź, Banacha 12/16, 90-237 Łódź, Poland.
Int J Mol Sci. 2021 Mar 30;22(7):3607. doi: 10.3390/ijms22073607.
In this research, we prepared foam scaffolds based on poly(l-lactide) (PLLA) and apatite whiskers (HAP) using thermally induced phase separation technique supported by the salt leaching process (TIPS-SL). Using sodium chloride having a size of (a) 150-315 μm, (b) 315-400 μm, and (c) 500-600 μm, three types of foams with different pore sizes have been obtained. Internal structure of the obtained materials has been investigated using SEM as well as μCT. The materials have been studied by means of porosity, density, and compression tests. As the most promising, the composite prepared with salt size of 500-600 μm was prepared also with the l-lysine modified apatite. The osteoblast hFOB 1.19 cell response for the scaffolds was also investigated by means of cell viability, proliferation, adhesion/penetration, and biomineralization. Direct contact cytotoxicity assay showed the cytocompatibility of the scaffolds. All types of foam scaffolds containing HAP whiskers, regardless the pore size or l-lysine modification induced significant stimulatory effect on the cal-cium deposits formation in osteoblasts. The PLLA/HAP scaffolds modified with l-lysine stimulated hFOB 1.19 osteoblasts proliferation. Compared to the scaffolds with smaller pores (150-315 µm and 315-400 µm), the PLLA/HAP foams with large pores (500-600 µm) promoted more effective ad-hesion of osteoblasts to the surface of the biomaterial.
在本研究中，我们采用盐析法辅助的热致相分离技术（TIPS-SL），制备了基于聚左旋乳酸（PLLA）和磷灰石晶须（HAP）的泡沫支架。使用粒径为（a）150 - 315μm、（b）315 - 400μm和（c）500 - 600μm的氯化钠，获得了三种不同孔径的泡沫材料。利用扫描电子显微镜（SEM）以及显微计算机断层扫描（μCT）研究了所得材料的内部结构。通过孔隙率、密度和压缩试验对材料进行了研究。作为最具前景的材料，还制备了用L-赖氨酸修饰磷灰石且盐粒径为500 - 600μm的复合材料。还通过细胞活力、增殖、黏附/穿透和生物矿化等方法研究了成骨细胞hFOB 1.19对支架的细胞反应。直接接触细胞毒性试验显示了支架的细胞相容性。所有类型含有HAP晶须的泡沫支架，无论孔径大小或L-赖氨酸修饰如何，均对成骨细胞中钙沉积物的形成产生显著的刺激作用。用L-赖氨酸修饰的PLLA/HAP支架刺激了hFOB 1.19成骨细胞的增殖。与具有较小孔径（150 - 315μm和315 - 400μm）的支架相比，具有大孔径（500 - 600μm）的PLLA/HAP泡沫促进了成骨细胞更有效地黏附到生物材料表面。
