Fletes-Vargas Gabriela, Espinosa-Andrews Hugo, Cervantes-Uc José Manuel, Limón-Rocha Isaías, Luna-Bárcenas Gabriel, Vázquez-Lepe Milton, Morales-Hernández Norma, Jiménez-Ávalos Jorge Armando, Mejía-Torres Dante Guillermo, Ramos-Martínez Paris, Rodríguez-Rodríguez Rogelio
Tecnología de Alimentos, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C (CIATEJ, A.C), Camino Arenero 1227, El Bajío del Arenal, Zapopan 45019, Jalisco, Mexico.
Departamento de Ciencias Clínicas, Centro Universitario de los Altos (CUALTOS), Universidad de Guadalajara, Carretera Tepatitlán Yahualica de González Gallo, Tepatitlan de Morelos 47620, Jalisco, Mexico.
Polymers (Basel). 2023 May 6;15(9):2203. doi: 10.3390/polym15092203.
Chitosan hydrogels are biomaterials with excellent potential for biomedical applications. In this study, chitosan hydrogels were prepared at different concentrations and molecular weights by freeze-drying. The chitosan sponges were physically crosslinked using sodium bicarbonate as a crosslinking agent. The X-ray spectroscopy (XPS and XRD diffraction), equilibrium water content, microstructural morphology (confocal microscopy), rheological properties (temperature sweep test), and cytotoxicity of the chitosan hydrogels (MTT assay) were investigated. XPS analysis confirmed that the chitosan hydrogels obtained were physically crosslinked using sodium bicarbonate. The chitosan samples displayed a semi-crystalline nature and a highly porous structure with mean pore size between 115.7 ± 20.5 and 156.3 ± 21.8 µm. In addition, the chitosan hydrogels exhibited high water absorption, showing equilibrium water content values from 23 to 30 times their mass in PBS buffer and high thermal stability from 5 to 60 °C. Also, chitosan hydrogels were non-cytotoxic, obtaining cell viability values ≥ 100% for the HT29 cells. Thus, physically crosslinked chitosan hydrogels can be great candidates as biomaterials for biomedical applications.
壳聚糖水凝胶是具有优异生物医学应用潜力的生物材料。在本研究中，通过冷冻干燥制备了不同浓度和分子量的壳聚糖水凝胶。壳聚糖海绵以碳酸氢钠作为交联剂进行物理交联。研究了壳聚糖水凝胶的X射线光谱（XPS和XRD衍射）、平衡含水量、微观结构形态（共聚焦显微镜）、流变学性质（温度扫描测试）以及细胞毒性（MTT法）。XPS分析证实所制备的壳聚糖水凝胶是通过碳酸氢钠进行物理交联的。壳聚糖样品呈现半结晶性质和高度多孔结构，平均孔径在115.7±20.5至156.3±21.8 µm之间。此外，壳聚糖水凝胶具有高吸水性，在PBS缓冲液中的平衡含水量值为其质量的23至30倍，并且在5至60°C范围内具有高热稳定性。而且，壳聚糖水凝胶无细胞毒性，对于HT29细胞获得的细胞活力值≥100%。因此，物理交联的壳聚糖水凝胶可成为生物医学应用生物材料的理想候选者。
