Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao, 266003, China.
Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266237, China.
Mar Biotechnol (NY). 2024 Jun;26(3):460-474. doi: 10.1007/s10126-024-10309-9. Epub 2024 Apr 13.
Colorful shells in bivalves are mostly caused by the presence of biological pigments, among which melanin is a key component in the formation of shell colours. Cyclic adenosine monophosphate (cAMP) is an important messenger in the regulation of pigmentation in some species. However, the role of cAMP in bivalve melanogenesis has not yet been reported. In this study, we performed in vitro and in vivo experiments to determine the role of cAMP in regulating melanogenesis in Pacific oysters. Besides, the function of cAMP-responsive element modulator (CREM) and the interactions between CREM and melanogenic genes were investigated. Our results showed that a high level of cAMP promotes the expression of melanogenic genes in Pacific oysters. CREM controls the expression of the MITF gene under cAMP regulation. In addition, CREM can regulate melanogenic gene expression, tyrosine metabolism, and melanin synthesis. These results indicate that cAMP plays an important role in the regulation of melanogenesis in Pacific oysters. CREM is a key transcription factor in the oyster melanin synthesis pathway, which plays a crucial role in oyster melanin synthesis through a cAMP-mediated CREM-MITF-TYR axis.
双壳贝类中的彩色贝壳主要是由生物色素的存在引起的，其中黑色素是贝壳颜色形成的关键成分。环磷酸腺苷（cAMP）是一些物种中色素沉着调节的重要信使。然而，cAMP 在双壳类动物黑色素生成中的作用尚未被报道。在这项研究中，我们进行了体外和体内实验，以确定 cAMP 在调节太平洋牡蛎黑色素生成中的作用。此外，还研究了 cAMP 反应元件调节剂（CREM）的功能以及 CREM 与黑色素生成基因之间的相互作用。我们的结果表明，高水平的 cAMP 促进了太平洋牡蛎中黑色素生成基因的表达。CREM 在 cAMP 调节下控制 MITF 基因的表达。此外，CREM 可以调节黑色素生成基因的表达、酪氨酸代谢和黑色素合成。这些结果表明 cAMP 在调节太平洋牡蛎黑色素生成中起着重要作用。CREM 是牡蛎黑色素合成途径中的关键转录因子，通过 cAMP 介导的 CREM-MITF-TYR 轴在牡蛎黑色素合成中发挥关键作用。
