Micronit B.V., Enschede, Netherlands.
Heriot-Watt University, Edinburgh, UK.
Methods Mol Biol. 2023;2679:163-180. doi: 10.1007/978-1-0716-3271-0_11.
Cell-free DNA has many applications in clinical medicine, in particular in cancer diagnosis and cancer treatment monitoring. Microfluidic-based solutions could provide solutions for rapid, cheaper, decentralized detection of cell-free tumoral DNA from a simple blood draw, or liquid biopsies, replacing invasive procedures or expensive scans. In this method, we present a simple microfluidic system for the extraction of cell-free DNA from low volume of plasma samples (≤500 μL). The technique is suitable for either static or continuous flow systems and can be used as a stand-alone module or integrated within a lab-on-chip system. The system relies on a simple yet highly versatile bubble-based micromixer module whose custom components can be fabricated with a combination of low-cost rapid prototyping techniques or ordered via widely available 3D-printing services. This system is capable of performing cell-free DNA extractions from small volumes of blood plasma with up to a tenfold increase in capture efficiency when compared to control methods.
无细胞游离 DNA 在临床医学中有许多应用，特别是在癌症诊断和癌症治疗监测方面。基于微流控的解决方案可以为从简单的血液抽取或液体活检中快速、更便宜、分散式地检测无细胞肿瘤 DNA 提供解决方案，从而替代侵入性程序或昂贵的扫描。在这种方法中，我们提出了一种简单的微流控系统，用于从低体积的血浆样本（≤500 μL）中提取无细胞游离 DNA。该技术适用于静态或连续流动系统，可作为独立模块使用，也可集成在芯片实验室系统中。该系统依赖于一个简单但功能非常强大的基于气泡的微混合器模块，其定制组件可以使用低成本快速原型制作技术组合进行制造，也可以通过广泛可用的 3D 打印服务进行订购。与对照方法相比，该系统能够从小体积的血液血浆中提取无细胞游离 DNA，捕获效率提高了十倍。
