与伤口愈合：体外研究。
Methanolic extract of and wound healing: an in vitro study.
机构信息
Department of Nuclear Applications, Institute of Nuclear Sciences, Ege University, Bornova, Izmir, 35100, Turkey.
Molecular Imaging Innovations Institute, Department of Radiology, Weill Cornell Medicine, New York, NY 10021, US.
出版信息
J Wound Care. 2023 Jun 2;32(6):392-398. doi: 10.12968/jowc.2023.32.6.392.
OBJECTIVE
(Lam.) Benth. (Bignoniaceae) syn. (Jacq. DC) is a tropical plant that is native to tropical Africa. The aim of this study was to determine if a methanolic extract prepared from (KAE) can promote wound healing in treated human normal epidermal keratinocyte (HaCaT) cells and human normal foreskin fibroblast cell line (BJ) cells compared with untreated cells.
METHOD
Experimental steps included: the methanolic extraction of the leaf and fruit of the plant; the preparation of HaCaT and BJ cell lines; cell culture with a stable tetrazolium salt-based proliferation assay; and the evaluation of the wound healing effect of KAE (2μg/ml) in BJ and HaCaT cells. The phytochemical contents of KAE were determined using liquid chromatography quadrupole time-of-flight mass spectrometry.
RESULTS
The following molecules were identified as being present in the KAE, among others: cholesterol sulfate; lignoceric acid; embelin; isostearic acid; linoleic acid; dioctyl phthalate; arg-pro-thr; 15-methyl-15(S)-PGE1; sucrose; benzododecinium (Ajatin); and 9-Octadecenamide (oleamide). KAE effected faster wound healing in treated cells compared with untreated cells for both cell lines. HaCaT cells that had been mechanically injured and treated with KAE healed completely in 48 hours compared with 72 hours for untreated HaCaT cells. Treated BJ cells healed completely in 72 hours compared with 96 hours for untreated BJ cells. Concentrations of KAE up to 300μg/ml had a very low cytotoxic effect on treated BJ and HaCaT cells.
CONCLUSION
The experimental data in this study support the potential of KAE-based wound healing treatment to accelerate wound healing.
目的
（Lam.）Benth.（Bignoniaceae）又名（Jacq. DC）是一种原产于热带非洲的热带植物。本研究旨在确定从（KAE）制备的甲醇提取物是否可以促进经处理的人正常表皮角质形成细胞（HaCaT）和人正常包皮成纤维细胞系（BJ）细胞的伤口愈合，与未经处理的细胞相比。
方法
实验步骤包括：植物的叶和果实的甲醇提取；HaCaT 和 BJ 细胞系的制备；用稳定的四唑盐增殖测定法进行细胞培养；以及评价 KAE（2μg/ml）在 BJ 和 HaCaT 细胞中的伤口愈合作用。使用液相色谱-四极杆飞行时间质谱法确定 KAE 的植物化学成分。
结果
KAE 中除其他物质外，还存在以下分子：胆固醇硫酸盐；愈创木酸；棓酸；异硬脂酸；亚油酸；邻苯二甲酸二辛酯；精氨酸-脯氨酸-苏氨酸；15-甲基-15（S）-PGE1；蔗糖；苯并多辛（Ajatin）；和 9-十八烯酰胺（油酰胺）。与未经处理的细胞相比，两种细胞系的 KAE 处理的细胞的伤口愈合更快。用 KAE 处理的机械损伤的 HaCaT 细胞在 48 小时内完全愈合，而未经处理的 HaCaT 细胞则需要 72 小时。用 KAE 处理的 BJ 细胞在 72 小时内完全愈合，而未经处理的 BJ 细胞则需要 96 小时。KAE 的浓度高达 300μg/ml 对处理过的 BJ 和 HaCaT 细胞的细胞毒性非常低。
结论
本研究的实验数据支持基于 KAE 的伤口愈合治疗有潜力加速伤口愈合。
Zotero 插件