Yang Woong-Suk, Lee Sung Ryul, Jeong Yong Joon, Park Dae Won, Cho Young Mi, Joo Hae Mi, Kim Inhye, Seu Young-Bae, Sohn Eun-Hwa, Kang Se Chan
School of Life Sciences and Biotechnology, Kyungpook National University , Daegu 41566, Republic of Korea.
Cardiovascular and Metabolic Disease Center and Department of Health Sciences and Technology, Inje University , Busan 47392, Republic of Korea.
J Agric Food Chem. 2016 May 11;64(18):3564-73. doi: 10.1021/acs.jafc.6b00425. Epub 2016 Apr 26.
The antiallergic potential of Arctium lappa L. was investigated in Sprague-Dawley rats, ICR mice, and RBL-2H3 cells. Ethanol extract (90%) of A. lappa (ALE, 100 μg/mL) inhibited the degranulation rate by 52.9%, determined by the level of β-hexosaminidase. ALE suppressed passive cutaneous anaphylaxis (PCA) in rats and attenuated anaphylaxis and histamine release in mice. To identify the active compound of ALE, we subsequently fractionated and determined the level of β-hexosaminidase in all subfractions. Oleamide was identified as an active compound of ALE, which attenuated the secretion of histamine and the production of tumor necrosis factor (TNF)-α and interleukin-4 (IL-4) in cells treated with compound 48/80 or A23187/phorbol myristate acetate (PMA). Oleamide suppressed FcεRI-tyrosine kinase Lyn-mediated pathway, c-Jun N-terminal kinases (JNK/SAPK), and p38 mitogen-activated protein kinases (p38-MAPKs). These results showed that ALE and oleamide attenuated allergic reactions and should serve as a platform to search for compounds with antiallergic activity.
在斯普拉格-道利大鼠、ICR小鼠和RBL-2H3细胞中研究了牛蒡的抗过敏潜力。牛蒡乙醇提取物（90%）（ALE，100μg/mL）通过β-己糖胺酶水平测定，使脱粒率降低了52.9%。ALE抑制了大鼠的被动皮肤过敏反应（PCA），并减轻了小鼠的过敏反应和组胺释放。为了鉴定ALE的活性化合物，我们随后对所有亚组分进行了分级分离并测定了β-己糖胺酶水平。油酸酰胺被鉴定为ALE的活性化合物，它减弱了用化合物48/80或A23187/佛波醇肉豆蔻酸酯乙酸酯（PMA）处理的细胞中组胺的分泌以及肿瘤坏死因子（TNF）-α和白细胞介素-4（IL-4）的产生。油酸酰胺抑制了FcεRI-酪氨酸激酶Lyn介导的途径、c-Jun氨基末端激酶（JNK/SAPK）和p38丝裂原活化蛋白激酶（p38-MAPK）。这些结果表明，ALE和油酸酰胺减轻了过敏反应，应作为寻找具有抗过敏活性化合物的平台。
