Departamento de Engenharia de Pesca, Universidade Federal do Ceará, Campus do Pici, Fortaleza, Ceará, Brazil.
Departamento de Patologia e Medicina Legal, Universidade Federal do Ceará, Campus do Porangabuçu, Fortaleza, Ceará, Brazil.
Biochimie. 2023 Nov;214(Pt B):61-76. doi: 10.1016/j.biochi.2023.05.016. Epub 2023 Jun 8.
The SfL-1 isoform from the marine red algae Solieria filiformis was produced in recombinant form (rSfL-1) and showed hemagglutinating activity and inhibition similar to native SfL. The analysis of circular dichroism revealed the predominance of β-strands structures with spectra of βI-proteins for both lectins, which had Melting Temperature (Tm) between 41 °C and 53 °C. The three-dimensional structure of the rSfL-1 was determined by X-ray crystallography, revealing that it is composed of two β-barrel domains formed by five antiparallel β chains linked by a short peptide between the β-barrels. SfL and rSfL-1 were able to agglutinate strains of Escherichia coli and Staphylococcus aureus and did not show antibacterial activity. However, SfL induced a reduction in E. coli biomass at concentrations from 250 to 125 μg mL, whereas rSfL-1 induced reduction in all concentrations tested. Additionally, rSfL-1 at concentrations from 250 to 62.5 μg mL, showed a statistically significant reduction in the number of colony-forming units, which was not noticed for SfL. Wound healing assay showed that the treatments with SfL and rSfL-1 act in reducing the inflammatory response and in the activation and proliferation of fibroblasts by a larger and fast deposition of collagen.
海洋红藻索氏马尾藻的 SfL-1 同工型以重组形式（rSfL-1）产生，表现出与天然 SfL 相似的血凝活性和抑制作用。圆二色性分析表明，两种凝集素都具有 βI 蛋白的 β 链结构优势，其熔点（Tm）在 41°C 和 53°C 之间。rSfL-1 的三维结构通过 X 射线晶体学确定，表明它由两个由五个反平行β链组成的β-桶结构域组成，β-桶之间由短肽连接。SfL 和 rSfL-1 能够凝集大肠杆菌和金黄色葡萄球菌菌株，但没有抗菌活性。然而，SfL 在 250 至 125μg/mL 的浓度下诱导大肠杆菌生物量减少，而 rSfL-1 在所有测试浓度下都诱导减少。此外，rSfL-1 在 250 至 62.5μg/mL 的浓度下，菌落形成单位数量呈统计学显著减少，而 SfL 则没有注意到这种情况。伤口愈合试验表明，SfL 和 rSfL-1 的处理通过胶原蛋白的大量快速沉积，在减少炎症反应和激活和增殖成纤维细胞方面起作用。
