Monteiro Abreu Ticiana, Castelo Melo Silva Luana Maria, Vanderlei Edfranck Sousa Oliveira, de Melo Cristiane Moutinho Lagos, Pereira Valeria Rego Alves, Barros Benevides Norma Maria
Departamento de Imunologia, Centro de Pesquisas Aggeu Magalhaes/FIOCRUZ, Av. Moraes Rego s/n, Cidade Universitaria, 50670-420 Recife, PE, Brazil.
Protein Pept Lett. 2012 Sep;19(9):975-81. doi: 10.2174/092986612802084474.
Marine algae can serve as sources of bioactive compounds and currently have been shown their potential biological and pharmaceutical applications. Marine algae lectins have been shown to be effective at controlling inflammatory processes. This work aimed to analyze the immunostimulatory properties of lectins from the marine algae Solieria filiformis (SfL), Pterocladiella capillacea (PcL) and Caulerpa cupressoides (CcL). This analysis was performed on BALB/c mouse splenocytes by measuring cytokine and nitric oxide production and cellular damage using tests of cytotoxicity and cell viability. These lectins were not cytotoxic (1-100 μg/mL), and were not able to induce IFN-γ and IL-2 production. IL- 10 production was induced at high levels by all lectins tested. Treatment with SfL induced IL-6 production at higher levels at all experimental times, whereas treatment with PcL and CcL induced higher levels only in 24 and 72 h. Treatment with SfL did not result in nitrite oxide production, whereas treatment with PcL or CcL was able to induce nitrite release at high levels (after 24, 48 and 72 h). Lesser cellular damage (5%) was observed in splenocytes treated with these lectins (10 μg/mL). Thus, the lectins from these algae were not cytotoxic, promoted increased in cell viability and induced Th2 immune responses in mouse splenocytes, indicating that they have anti-inflammatory effects.
海洋藻类可作为生物活性化合物的来源，目前已显示出其潜在的生物学和药物应用价值。海洋藻类凝集素已被证明在控制炎症过程中有效。本研究旨在分析丝状索藻（SfL）、毛细茎羽藻（PcL）和柏状蕨藻（CcL）这三种海洋藻类凝集素的免疫刺激特性。通过使用细胞毒性和细胞活力测试来测量细胞因子和一氧化氮的产生以及细胞损伤，对BALB/c小鼠脾细胞进行了该分析。这些凝集素（1-100μg/mL）无细胞毒性，且不能诱导IFN-γ和IL-2的产生。所有测试的凝集素均能高水平诱导IL-10的产生。在所有实验时间点，SfL处理均能诱导更高水平的IL-6产生，而PcL和CcL处理仅在24小时和72小时诱导更高水平的IL-6产生。SfL处理未导致亚硝酸盐产生，而PcL或CcL处理能够在24、48和72小时后高水平诱导亚硝酸盐释放。在用这些凝集素（10μg/mL）处理的脾细胞中观察到较小的细胞损伤（5%）。因此，这些藻类的凝集素无细胞毒性，能促进细胞活力增加，并在小鼠脾细胞中诱导Th2免疫反应，表明它们具有抗炎作用。
