Laboratório de Biotecnologia Marinha - BioMar-Lab, Departamento de Engenharia de Pesca, Universidade Federal do Ceará, Campus do Pici s/n, bloco 871, 60440-900 Fortaleza, Ceará, Brazil.
Laboratório de Biotecnologia Marinha - BioMar-Lab, Departamento de Engenharia de Pesca, Universidade Federal do Ceará, Campus do Pici s/n, bloco 871, 60440-900 Fortaleza, Ceará, Brazil; Laboratório Integrado de Biomoléculas - LIBS, Departamento de Patologia e Medicina Legal, Universidade Federal do Ceará, Monsenhor Furtado, s/n, 60430-160 Fortaleza, Ceará, Brazil.
Int J Biol Macromol. 2018 Feb;107(Pt A):1320-1329. doi: 10.1016/j.ijbiomac.2017.09.116. Epub 2017 Sep 29.
As described in the literature, Solieria filiformis lectin (SfL) from the marine red alga S. filiformis was found to have antinociceptive and anti-inflammatory effects. In this study, we characterized two SfL variants, SfL-1 and SfL-2, with molecular mass of 27,552Da and 27,985Da, respectively. The primary structures of SfL-1 and SfL-2 consist of four tandem-repeat protein domains with 67 amino acids each. SfL-1 and -2 showed high similarity to OAAH-family lectins. 3D structure prediction revealed that SfL-1 and -2 are composed of two β-barrel-like domains formed by five antiparallel β-strands, which are connected by a short peptide linker. Furthermore, the mixture of isoforms (SfLs) showed anticancer effect against MCF-7 cells. Specifically, SfLs inhibited 50% of viability in MCF-7 cells after treatment at 125μg.mL, while the inhibition of Human Dermal Fibroblasts (HDF) was 34% with the same treatment. Finally, 24h after treatment, 25% of MCF-7 cells were in early apoptosis and 35% in late apoptosis. Evaluation of pro- and anti-apoptotic gene expression of MCF-7 cells revealed that SfLs induced caspase-dependent apoptosis within 24h.
如文献所述，从海洋红藻 Solieria filiformis 中分离出的 Solieria filiformis 凝集素 (SfL) 具有镇痛和抗炎作用。在这项研究中，我们对两种 SfL 变体 SfL-1 和 SfL-2 进行了表征，它们的分子量分别为 27552Da 和 27985Da。SfL-1 和 SfL-2 的一级结构由四个串联重复的蛋白结构域组成，每个结构域含有 67 个氨基酸。SfL-1 和 -2 与 OAAH 家族凝集素具有高度相似性。3D 结构预测表明 SfL-1 和 -2 由两个由五个反平行 β-折叠组成的 β-桶样结构域组成，由短肽连接子连接。此外，同工型混合物 (SfLs) 对 MCF-7 细胞具有抗癌作用。具体来说，SfLs 在 125μg.mL 处理后对 MCF-7 细胞的活力抑制了 50%，而相同处理对人真皮成纤维细胞 (HDF) 的抑制率为 34%。最后，处理 24 小时后，25%的 MCF-7 细胞处于早期凋亡，35%处于晚期凋亡。对 MCF-7 细胞中促凋亡和抗凋亡基因表达的评估表明，SfLs 在 24 小时内诱导了 caspase 依赖性凋亡。
