灵芝三萜对肝癌生长和转移的抑制作用。
Inhibitory effects of Ganoderma lucidum triterpenoid on the growth and metastasis of hepatocellular carcinoma.
作者信息
Ding Zhuyuan, Zhou Ziyi, Cheng Xinge, Wang Houli, Liu Jiayi, Cai Yeyu, Liu Huan, Lv Min, Pan Yuning, Xiao Enhua
机构信息
Department of Imaging, Ningbo First Hospital Ningbo 315000, Zhejiang, China.
Department of Radiology, Maternal and Child Health Hospital of Hubei Province Wuhan 430070, Hubei, China.
出版信息
Am J Transl Res. 2023 May 15;15(5):3410-3423. eCollection 2023.
OBJECTIVE
To investigate the inhibitory effects and mechanisms of triterpenoids from Ganoderma lucidum (G. lucidum triterpenoids) on the growth and metastasis of hepatocellular carcinoma (HCC) both and .
METHODS
In experiments, the inhibitory effects of G. lucidum triterpenoids on human HCC SMMC-7721 cell lines were investigated by observing the proliferation, apoptosis, migration and invasion phenotypes of the cell line and assessing the cell cycles as well as the cell apoptosis and proliferation. In experiments, nude mouse SMMC-7721 tumor models were established and divided into control group, treatment group A (low concentration group) and treatment group B (high concentration group) according to the treatment models received. Magnetic resonance imaging (MRI) was performed 3 times on each mouse model to calculate their tumor volumes. The liver and kidney functions of the models were evaluated. Tissues harvested from their solid organs were subjected to HE staining, and the tumor tissues were subjected to HE staining and immunohistochemical staining (E-cad, Ki-67, and Tunel), respectively.
RESULTS
i. In experiments, G. lucidum triterpenoids could inhibit the growth of human HCC SMMC-7721 cell lines via regulating their proliferation and apoptosis phenotype. ii. In experiments, the comparison of tumor volumes of mouse models obtained from the second and third MIR scanning was found to be statistically significant between the control group and treatment group A (P<0.05); and statistically significant differences were also found in the tumor volumes from the second and third MRI scanning between the control group and treatment group B (P<0.05). iii. No significant acute injuries or adverse effects were observed in the liver or kidney of the nude mice.
CONCLUSION
G. lucidum triterpenoids could inhibit the growth of tumor cells via blocking their proliferation, accelerating apoptosis, and inhibiting migration and invasion, without marked toxic effects on normal organs and tissues in the body.
目的
研究灵芝三萜对肝癌（HCC）生长和转移的抑制作用及其机制。
方法
体外实验中，通过观察人肝癌SMMC - 7721细胞系的增殖、凋亡、迁移和侵袭表型，并评估细胞周期以及细胞凋亡和增殖情况，研究灵芝三萜对该细胞系的抑制作用。体内实验中，建立裸鼠SMMC - 7721肿瘤模型，并根据接受的治疗模式分为对照组、治疗组A（低浓度组）和治疗组B（高浓度组）。对每个小鼠模型进行3次磁共振成像（MRI）以计算其肿瘤体积。评估模型的肝肾功能。从其实体器官采集的组织进行苏木精 - 伊红（HE）染色，肿瘤组织分别进行HE染色和免疫组织化学染色（E - cad、Ki - 67和Tunel）。
结果
i. 体外实验中，灵芝三萜可通过调节人肝癌SMMC - 7721细胞系的增殖和凋亡表型来抑制其生长。ii. 体内实验中，发现对照组与治疗组A第二次和第三次MRI扫描获得的小鼠模型肿瘤体积比较有统计学意义（P<0.05）；对照组与治疗组B第二次和第三次MRI扫描的肿瘤体积也有统计学显著差异（P<0.05）。iii. 在裸鼠的肝脏或肾脏中未观察到明显的急性损伤或不良反应。
结论
灵芝三萜可通过阻断肿瘤细胞增殖、加速凋亡以及抑制迁移和侵袭来抑制肿瘤细胞生长，且对体内正常器官和组织无明显毒性作用。
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