Shenzhen Baoan Traditional Chinese Medicine Hospital Group, Shenzhen, People's Republic of China.
Department of Pharmacy, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, People's Republic of China.
Nat Prod Res. 2022 Aug;36(16):4125-4131. doi: 10.1080/14786419.2021.1976175. Epub 2021 Sep 20.
() is a famous medicinal fungus used as a traditional medicine for generations in China. Among bioactivities that G. lucidum possesses, the anti-tumor effect has aroused extensive interests. In this study, one new triterpene () was isolated from 90% ethanol extract of the dried fruiting bodies of . Its structure was determined based on the analysis of its spectroscopic data, including 1 D and 2 D NMR and MS spectra. Furthermore, elicited moderate anti-tumor activities (IC values of 15.38 ± 0.34 and 18.61 ± 0.55 μM for A549 and HepG2 cells, respectively) compared with cisplatin which was employed as the positive drug with IC values of 8.21 ± 0.17 and 5.36 ± 0.29 μM for A549 and HepG2 cells, respectively. The mechanism study by RT-qPCR and western blot analysis suggested that the p53/caspase-3 pathway is involved in the -induced apoptosis.
() 是一种著名的药用真菌，在中国被世代用作传统药物。在灵芝具有的多种生物活性中，其抗肿瘤作用引起了广泛的兴趣。在这项研究中，从干燥的灵芝子实体 90%乙醇提取物中分离得到一种新的三萜（）。根据其光谱数据分析，包括 1D 和 2D NMR 和 MS 光谱，确定了其结构。此外，与顺铂（阳性药物，A549 和 HepG2 细胞的 IC 值分别为 8.21 ± 0.17 和 5.36 ± 0.29 μM）相比，（）对 A549 和 HepG2 细胞表现出中等的抗肿瘤活性（IC 值分别为 15.38 ± 0.34 和 18.61 ± 0.55 μM）。通过 RT-qPCR 和 Western blot 分析的机制研究表明，p53/caspase-3 途径参与了诱导的细胞凋亡。
