BACKGROUND

Due to the increasing prevalence of oral squamous cell carcinoma (OSCC) in Iran and especially in young people, this study aimed to identify human papillomavirus (HPV) using p16 in OSCC.

MATERIALS AND METHODS

In this descriptive-analytical cross-sectional study, 40 samples from the archives of the Pathology Department of Kashani Hospital were selected by a definitive diagnosis of OSCC with neck dissection. Demographic information including age, gender, location, and size of the lesion was obtained. Samples were divided into two groups based on lymph node (LN) metastasis. Immunohistochemical staining was performed for p16. Data were entered into SPSS 24 software and statistically analyzed by -test, ANOVA, and Spearman nonparametric test. <0.05 was statistically significant.

RESULTS

The mean age of patients was 59.7 ± 17.11 which in terms of age and gender there was no significant difference between the two groups including with and without cervical LN metastasis ( > 0.05). There was no significant difference between the two groups based on the grade of tumor, perninural invasion, tumor size and location ( > 0.05). The only significant difference between the two groups was based on lymphovascular invasion and disease stage ( < 0.05). The p16 expression also showed a significant difference between the two groups ( < 0.05).

CONCLUSION

In OSCCs without cervical LN metastasis, a significant increase in p16 expression was observed compared to samples with cervical LNs metastasis. The presence of HPV was higher in samples with less LNs metastasis and possibly a better prognosis.