Xiang Mingjie, Liu Shengjie, Wang Xiaoting, Zhang Mingming, Yan Weiyi, Wu Jianhui, Wang Qilin, Li Chunlian, Zheng Weijun, He Yilin, Ge Yunxia, Wang Changfa, Kang Zhensheng, Han Dejun, Zeng Qingdong
State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F University, Yangling, 712100 Shaanxi China.
State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A&F University, Yangling, 712100 Shaanxi China.
Mol Breed. 2023 Feb 9;43(2):13. doi: 10.1007/s11032-023-01359-3. eCollection 2023 Feb.
Wheat is an essential food crop and its high and stable yield is suffering from great challenges due to the limitations of current breeding technology and various stresses. Accelerating molecularly assisted stress-resistance breeding is critical. Through a meta-analysis of published loci in wheat over the last two decades, we selected 60 loci with main breeding objectives, high heritability, and reliable genotyping, such as stress resistance, yield, plant height, and resistance to spike germination. Then, using genotyping by target sequencing (GBTS) technology, we developed a liquid phase chip based on 101 functional or closely linked markers. The genotyping of 42 loci was confirmed in an extensive collection of Chinese wheat cultivars, indicating that the chip can be used in molecular-assisted selection (MAS) for target breeding goals. Besides, we can perform the preliminary parentage analysis with the genotype data. The most significant contribution of this work lies in translating a large number of molecular markers into a viable chip and providing reliable genotypes. Breeders can quickly screen germplasm resources, parental breeding materials, and intermediate materials for the presence of excellent allelic variants using the genotyping data by this chip, which is high throughput, convenient, reliable, and cost-efficient.
The online version contains supplementary material available at 10.1007/s11032-023-01359-3.
小麦是一种重要的粮食作物，由于当前育种技术的局限性和各种胁迫，其高产稳产正面临巨大挑战。加速分子辅助抗逆育种至关重要。通过对过去二十年发表的小麦基因座进行荟萃分析，我们选择了60个具有主要育种目标、高遗传力和可靠基因分型的基因座，如抗逆性、产量、株高和抗穗发芽性。然后，利用靶向测序基因分型（GBTS）技术，我们基于101个功能或紧密连锁标记开发了一种液相芯片。在大量中国小麦品种中证实了42个基因座的基因分型，表明该芯片可用于目标育种目标的分子辅助选择（MAS）。此外，我们可以利用基因型数据进行初步的亲缘关系分析。这项工作最重要的贡献在于将大量分子标记转化为一种可行的芯片，并提供可靠的基因型。育种者可以利用该芯片的基因分型数据快速筛选种质资源、亲本育种材料和中间材料，以确定是否存在优良等位变异，该芯片具有高通量、方便、可靠且成本效益高的特点。
在线版本包含可在10.1007/s11032-023-01359-3获取的补充材料。
