Liu Shengjie, Wang Xiaoting, Zhang Yayun, Jin Yangang, Xia Zhonghua, Xiang Mingjie, Huang Shuo, Qiao Linyi, Zheng Weijun, Zeng Qingdong, Wang Qilin, Yu Rui, Singh Ravi P, Bhavani Sridhar, Kang Zhensheng, Han Dejun, Wang Changfa, Wu Jianhui
State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F University, Yangling, 712100, Shaanxi, People's Republic of China.
Jiangsu Ruihua Agricultural Science and Technology Co. Ltd, Suqian, 223800, Jiangsu, People's Republic of China.
Theor Appl Genet. 2022 Jan;135(1):351-365. doi: 10.1007/s00122-021-03970-4. Epub 2021 Oct 19.
YrFDC12 and PbcFDC, co-segregated in chromosome 4BL, and significantly interacted with Yr30/Pbc1 to enhance stripe rust resistance and to promote pseudo-black chaff development. Cultivars with durable resistance are the most popular means to control wheat stripe rust. Durable resistance can be achieved by stacking multiple adult plant resistance (APR) genes that individually have relatively small effect. Chinese wheat cultivars Ruihua 520 (RH520) and Fengdecun 12 (FDC12) confer partial APR to stripe rust across environments. One hundred and seventy recombinant inbred lines from the cross RH520 × FDC12 were used to determine the genetic basis of resistance and identify genomic regions associated with stripe rust resistance. Genotyping was carried out using 55 K SNP array, and eight quantitative trait loci (QTL) were detected on chromosome arms 2AL, 2DS, 3BS, 4BL, 5BL (2), and 7BL (2) by inclusive composite interval mapping. Only QYr.nwafu-3BS from RH520 and QYr.nwafu-4BL.2 (named YrFDC12 for convenience) from FDC12 were consistent across the four testing environments. QYr.nwafu-3BS is likely the pleiotropic resistance gene Sr2/Yr30. YrFDC12 was mapped in a 2.1-cM interval corresponding to 12 Mb and flanked by SNP markers AX-111121224 and AX-89518393. Lines harboring both Yr30 and YrFDC12 displayed higher resistance than the parents and expressed pseudo-black chaff (PBC) controlled by loci Pbc1 and PbcFDC12, which co-segregated with Yr30 and YrFDC12, respectively. Both marker-based and pedigree-based kinship analyses revealed that YrFDC12 was inherited from founder parent Zhou 8425B. Fifty-four other wheat cultivars shared the YrFDC12 haplotype. These results suggest an effective pyramiding strategy to acquire highly effective, durable stripe rust resistance in breeding.
YrFDC12和PbcFDC在4B染色体上共分离，并与Yr30/Pbc1显著互作，增强了条锈病抗性并促进了伪黑颖壳的发育。具有持久抗性的品种是控制小麦条锈病最常用的手段。通过聚合多个单个效应相对较小的成株抗性（APR）基因可实现持久抗性。中国小麦品种瑞华520（RH520）和丰德存12（FDC12）在不同环境下对条锈病具有部分APR抗性。利用RH520×FDC12杂交产生的170个重组自交系来确定抗性的遗传基础，并鉴定与条锈病抗性相关的基因组区域。使用55K SNP芯片进行基因分型，通过完备复合区间作图在染色体臂2AL、2DS、3BS、4BL、5BL（2个）和7BL（2个）上检测到8个数量性状位点（QTL）。只有来自RH520的QYr.nwafu - 3BS和来自FDC12的QYr.nwafu - 4BL.2（为方便起见命名为YrFDC12）在四个测试环境中都一致。QYr.nwafu - 3BS可能是多效抗性基因Sr2/Yr30。YrFDC12定位在一个2.1厘摩的区间内，对应12兆碱基，两侧是SNP标记AX - 111121224和AX - 8951839。同时携带Yr30和YrFDC12的株系表现出比亲本更高的抗性，并表现出由分别与Yr30和YrFDC12共分离的位点Pbc1和PbcFDC12控制的伪黑颖壳（PBC）。基于标记和系谱的亲缘关系分析均表明YrFDC12是从奠基亲本周8425B遗传而来。另外54个小麦品种共享YrFDC12单倍型。这些结果表明了一种在育种中获得高效、持久条锈病抗性有效的聚合策略。
