Sheng Dece, Qiao Linyi, Zhang Xiaojun, Li Xin, Chang Lifang, Guo Huijuan, Zhang Shuwei, Chen Fang, Chang Zhijian
College of Life Science, Shanxi University, Taiyuan, 030006 Shanxi China.
College of Agronomy, Shanxi Province Key Laboratory of Crop Genetics and Gene Improvement, Shanxi Agricultural University, Taiyuan, 030031 Shanxi China.
Mol Breed. 2022 Aug 29;42(9):52. doi: 10.1007/s11032-022-01318-4. eCollection 2022 Sep.
Leaf rust (LR), caused by (), is one of the most important fungal diseases of wheat worldwide. The wheat accession CH1539 showed a high level of resistance to leaf rust. A mapping population of 184 recombinant inbred lines (RILs) was developed from a cross between the resistant accession CH1539 and the susceptible cultivar SY95-71. The RILs showed segregating infection responses to Eriks. () race THK at the seedling stage. Genetic analysis showed that leaf rust resistance was controlled by a monogenic gene, and the potential locus was temporarily named . Bulked segregant analysis (BSA) using a 35 K DArTseq array located on the short arm of chromosome 2B. Subsequently, a genetic linkage map of was constructed using the developed 2BS chromosome-specific markers, and its flanking markers were and . An F subpopulation with 3619 lines was constructed by crossing the resistant and susceptible lines selected from the RIL population. The inoculation identification results showed that was recessively inherited and was fine-mapped to a 779.4-kb region between markers and at the end of 2BS. The linkage marker analysis showed that the positions of and were the same, but the identification results of the resistance spectrum indicated that the causal genes of the two might be different. The resistant materials reported in this study and the cosegregation marker can be used for marker-assisted selection breeding of leaf rust-resistant wheat cultivars.
The online version contains supplementary material available at 10.1007/s11032-022-01318-4.
叶锈病（LR）由（）引起，是全球小麦最重要的真菌病害之一。小麦种质CH1539对叶锈病表现出高抗性。从抗性种质CH1539与感病品种SY95 - 71杂交后代中构建了一个包含184个重组自交系（RILs）的作图群体。这些RILs在苗期对Eriks.（）小种THK表现出分离的感染反应。遗传分析表明，叶锈病抗性由一个单基因控制，该潜在位点暂命名为（）。利用35K DArTseq芯片进行的混合分组分析法（BSA）将（）定位在2B染色体短臂上。随后，利用开发的2BS染色体特异性标记构建了（）的遗传连锁图谱，其侧翼标记为（）和（）。通过将从RIL群体中选出的抗性和感病品系杂交，构建了一个包含3619个株系的F亚群体。接种鉴定结果表明，（）是隐性遗传的，并被精细定位到2BS末端标记（）和（）之间779.4 kb的区域。连锁标记分析表明，（）和（）的位置相同，但抗性谱鉴定结果表明二者的致病基因可能不同。本研究报道的抗性材料和共分离标记可用于抗叶锈病小麦品种的分子标记辅助选择育种。
在线版本包含可在10.1007/s11032 - 022 - 01318 - 4获取的补充材料。
