Kassa Mulualem T, You Frank M, Hiebert Colin W, Pozniak Curtis J, Fobert Pierre R, Sharpe Andrew G, Menzies James G, Humphreys D Gavin, Rezac Harrison Nicole, Fellers John P, McCallum Brent D, McCartney Curt A
Agriculture and Agri-Food Canada, Morden Research and Development Centre, 101 Route 100, Morden, MB, R6M 1Y5, Canada.
National Research Council, 110 Gymnasium Place, Saskatoon, SK, S7N 0W9, Canada.
BMC Plant Biol. 2017 Feb 15;17(1):45. doi: 10.1186/s12870-017-0993-7.
Lr16 is a widely deployed leaf rust resistance gene in wheat (Triticum aestivum L.) that is highly effective against the North American Puccinia triticina population when pyramided with the gene Lr34. Lr16 is a seedling leaf rust resistance gene conditioning an incompatible interaction with a distinct necrotic ring surrounding the uredinium. Lr16 was previously mapped to the telomeric region of the short arm of wheat chromosome 2B. The goals of this study were to develop numerous single nucleotide polymorphism (SNP) markers for the Lr16 region and identify diagnostic gene-specific SNP marker assays for marker-assisted selection (MAS).
Forty-three SNP markers were developed and mapped on chromosome 2BS tightly linked with the resistance gene Lr16 across four mapping populations representing a total of 1528 gametes. Kompetitive Allele Specific PCR (KASP) assays were designed for all identified SNPs. Resistance gene analogs (RGAs) linked with the Lr16 locus were identified and RGA-based SNP markers were developed. The diagnostic potential of the SNPs co-segregating with Lr16 was evaluated in a diverse set of 133 cultivars and breeding lines. Six SNP markers were consistent with the Lr16 phenotype and are accurately predictive of Lr16 for all wheat lines/cultivars in the panel.
Lr16 was mapped relative to SNP markers in four populations. Six SNP markers exhibited high quality clustering in the KASP assay and are suitable for MAS of Lr16 in wheat breeding programs.
Lr16是小麦（Triticum aestivum L.）中广泛应用的抗叶锈病基因，当与Lr34基因聚合时，对北美小麦叶锈菌（Puccinia triticina）群体具有高效抗性。Lr16是一个幼苗抗叶锈病基因，与夏孢子堆周围明显的坏死环形成不亲和互作。Lr16先前被定位到小麦2B染色体短臂的端粒区域。本研究的目标是开发大量用于Lr16区域的单核苷酸多态性（SNP）标记，并鉴定用于标记辅助选择（MAS）的诊断性基因特异性SNP标记检测方法。
开发了43个SNP标记，并在代表总共1528个配子的四个作图群体中定位到与抗性基因Lr16紧密连锁的2BS染色体上。为所有鉴定出的SNP设计了竞争性等位基因特异性PCR（KASP）检测方法。鉴定了与Lr16基因座连锁的抗性基因类似物（RGA），并开发了基于RGA的SNP标记。在133个不同的品种和育种系中评估了与Lr16共分离的SNP的诊断潜力。六个SNP标记与Lr16表型一致，并且能够准确预测该面板中所有小麦品系/品种的Lr16。
在四个群体中相对于SNP标记对Lr16进行了定位。六个SNP标记在KASP检测中表现出高质量聚类，适用于小麦育种计划中Lr16的MAS。
