Ren Rui, Liu Wei, Yao Min, Jia Yuan, Huang Luyao, Li Wenqian, He Xin, Guan Mei, Liu Zhongsong, Guan Chunyun, Hua Wei, Xiong Xinghua, Qian Lunwen
Collaborative Innovation Center of Grain and Oil Crops in South China, Hunan Agricultural University, Changsha, 410128 China.
Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan, 430062 China.
Mol Breed. 2022 Oct 26;42(11):69. doi: 10.1007/s11032-022-01337-1. eCollection 2022 Nov.
Plant height is a key morphological trait in rapeseed, which not only plays an important role in determining plant architecture, but is also an important characteristic related to yield. Presently, the improvement of plant architecture is a major challenge in rapeseed breeding. This work was carried out to identify genetic loci related to plant height in rapeseed. In this study, a genome-wide association study (GWAS) of plant height was performed using a 60 K Illumina Infinium SNP array and 203 accessions. Eleven haplotypes containing important candidate genes were detected and significantly associated with plant height on chromosomes A02, A03, A05, A07, A08, C03, C06, and C09. Moreover, regional association analysis of 50 resequenced rapeseed inbred lines was used to further analyze these eleven haplotypes and revealed nucleotide variation in the - and - gene regions related to the phenotypic variation in plant height. Furthermore, coexpression network analysis showed that - and - were directly connected with hormone genes and transcription factors and formed a potential network regulating the plant height of rapeseed. Our results will aid in the development of haplotype functional markers to further improve plant height in rapeseed.
The online version contains supplementary material available at 10.1007/s11032-022-01337-1.
株高是油菜籽的一个关键形态性状，它不仅在决定植株结构方面起着重要作用，也是与产量相关的一个重要特征。目前，改善植株结构是油菜籽育种中的一项重大挑战。开展这项工作是为了鉴定油菜籽中与株高相关的基因位点。在本研究中，使用60K Illumina Infinium SNP芯片和203份材料对株高进行了全基因组关联研究（GWAS）。检测到11个包含重要候选基因的单倍型，它们与A02、A03、A05、A07、A08、C03、C06和C09染色体上的株高显著相关。此外，利用50个重测序油菜自交系的区域关联分析对这11个单倍型进行了进一步分析，揭示了与株高表型变异相关的-和-基因区域的核苷酸变异。此外，共表达网络分析表明-和-与激素基因和转录因子直接相连，形成了一个潜在的调控油菜株高的网络。我们的结果将有助于开发单倍型功能标记，以进一步提高油菜的株高。
在线版本包含可在10.1007/s11032-022-01337-1获取的补充材料。
