Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry and Materials Science, Northwest University, Xi'an 710127, China.
ACS Appl Mater Interfaces. 2023 Jun 28;15(25):30643-30652. doi: 10.1021/acsami.3c05025. Epub 2023 Jun 14.
In this work, a boronate affinity-functionalized hierarchical mesoporous metal-organic framework adsorbent with boronate sites only in the small mesopore has been structured based on UiO-66@FeO. The introduction of large mesopores in the adsorbent can promote the diffusion of small -diol-containing compounds (-diols) into small mesopore channels, and the removal of the adsorption sites on the external surface of materials and in large mesopores can enhance the size-exclusion effect of the adsorbent. In addition, the adsorbent has faster adsorption kinetics and excellent selectivity to small -diols. Finally, a magnetic dispersive solid-phase extraction coupled with high-performance liquid chromatography was established for the enrichment and detection of nucleotides in plasma. Four nucleotides achieve the recoveries from 93.25 to 118.79%, the limits of detection from 0.35 to 1.26 ng·mL, and the intra-day and inter-day relative standard deviations of less than 10.2%. In conclusion, this method can be directly used for the detection of small -diol targets in complex biological samples without protein precipitation prior to the extraction.
在这项工作中，基于 UiO-66@FeO 构建了一种硼酸亲和功能化的分级介孔金属有机骨架吸附剂，其硼酸基团仅位于小孔中。吸附剂中大孔的引入可以促进含有小分子二醇的化合物（小分子二醇）扩散进入小孔道，去除材料外表面和大孔中的吸附位点可以增强吸附剂的尺寸排阻效应。此外，该吸附剂对小分子二醇具有更快的吸附动力学和优异的选择性。最后，建立了一种基于磁性分散固相萃取与高效液相色谱联用的方法，用于血浆中核苷酸的富集和检测。四种核苷酸的回收率为 93.25%至 118.79%，检出限为 0.35 至 1.26ng·mL，日内和日间相对标准偏差均小于 10.2%。总之，该方法可以直接用于复杂生物样品中小分子二醇目标物的检测，无需在提取前进行蛋白质沉淀。
