Hatch G G, Anderson T M, Lubet R A, Kouri R E, Putman D L, Cameron J W, Nims R W, Most B, Spalding J W, Tennant R W
Environ Mutagen. 1986;8(4):515-31. doi: 10.1002/em.2860080404.
Twelve chemicals from diverse structural classes were tested under code for their capacity to enhance the transformation of Syrian hamster embryo cells by simian adenovirus SA7 in two independent laboratories. Pretreatment of hamster cells with eight of those chemicals (reserpine, dichlorvos, methapyrilene hydrochloride, benzidine dihydrochloride, diphenylhydantoin, cinnamyl anthranilate, 11-aminoundecanoic acid, and 4,4'-oxydianiline) produced repeatable enhancement of SA7 transformation at two or more consecutive dose levels, which constitutes clear evidence of enhancing activity in this assay. Both toxic and nontoxic doses of each of these chemicals caused enhancement of virus transformation. Two chemicals (2,6-dichloro-p-phenylenediamine and cinnamaldehyde) produced some evidence of enhancing activity (repeatable transformation enhancement at one dose). Dose ranges for cytotoxicity and enhancement of SA7 transformation were similar in both laboratories for all chemicals producing activity. The final two chemicals, chloramphenicol sodium succinate and ethylene thiourea, failed to reproducibly demonstrate either significant cytotoxicity or enhancement of SA7 transformation at concentrations up to 10-20 mM. The test results for these 12 chemicals were combined with the test results for 9 known carcinogens and noncarcinogens in order to evaluate relationships between activity, dose response, and lowest effective enhancing concentration for these compounds, as well as to correlate them with rodent carcinogenesis classifications. The Syrian hamster embryo cell-SA7 system demonstrated reproducible test responses in both intra- and interlaboratory studies and detected 13 out of 15 known rodent carcinogens.
在两个独立实验室中，对12种结构各异的化学物质进行了编码测试，以检测它们增强猿猴腺病毒SA7诱导叙利亚仓鼠胚胎细胞转化的能力。其中8种化学物质（利血平、敌敌畏、盐酸美吡拉敏、盐酸联苯胺、苯妥英、邻氨基苯甲酸肉桂酯、11-氨基十一酸和4,4'-二氨基二苯醚）对仓鼠细胞进行预处理后，在两个或更多连续剂量水平上均产生了可重复的SA7转化增强效果，这构成了该检测中增强活性的明确证据。这些化学物质的毒性和无毒剂量均能导致病毒转化增强。另外两种化学物质（2,6-二氯对苯二胺和肉桂醛）显示出一些增强活性的证据（在一个剂量下可重复的转化增强）。对于所有具有活性的化学物质，两个实验室中细胞毒性和SA7转化增强的剂量范围相似。最后两种化学物质，琥珀氯霉素钠和乙撑硫脲，在浓度高达10 - 20 mM时，未能重复显示出显著的细胞毒性或SA7转化增强。将这12种化学物质的测试结果与9种已知致癌物和非致癌物的测试结果相结合，以评估这些化合物的活性、剂量反应和最低有效增强浓度之间的关系，并将它们与啮齿动物致癌分类相关联。叙利亚仓鼠胚胎细胞 - SA7系统在实验室内和实验室间研究中均显示出可重复的测试反应，并检测出了15种已知啮齿动物致癌物中的13种。
