Tu A, Hallowell W, Pallotta S, Sivak A, Lubet R A, Curren R D, Avery M D, Jones C, Sedita B A, Huberman E
Environ Mutagen. 1986;8(1):77-98. doi: 10.1002/em.2860080108.
Three independent laboratories tested eight "model" and five coded chemicals in the Syrian hamster embryo clonal transformation assay system to establish the intra- and interlaboratory reproducibility of the system and to identify sources of variability. When a common cell pool and the same lot of fetal calf serum were used, the three laboratories obtained consensus on the activity of eight model chemicals: five chemicals (benzo(a)pyrene, 7,12-dimethylbenz(a)anthracene, N-methyl-N'-nitro-N-nitrosoguanidine, nitroquinoline-N-oxide, and lead chromate) induced morphological transformation without exogenous metabolic activation and three (N-2-fluorenylacetamide, pyrene, and anthracene) produced no transformation response. Five coded chemicals (2,6-dichloro p-phenylenediamine, 4,4'-oxydianiline, cinnamyl anthranilate, dichlorvos, and reserpine), representative of environmental chemical classes, but not necessarily strong carcinogens, produced more equivocal responses in this interlaboratory study. Thus, while the assay can be used to distinguish between transforming and nontransforming chemicals in some cases, the intrinsic limitations in low transformation frequency and in achieving any dose-response results are major constraints to the use of this system in a routine testing program at the present time. Efforts to increase the transformation frequency or to amplify the expression of the transformed phenotype constitute some of the approaches which should be explored in order to overcome these limitations.
三个独立实验室在叙利亚仓鼠胚胎克隆转化试验系统中对8种“标准”化学品和5种编码化学品进行了测试，以确定该系统在实验室内和实验室间的可重复性，并识别变异性来源。当使用共同的细胞库和同一批次的胎牛血清时，三个实验室就8种标准化学品的活性达成了共识：5种化学品（苯并(a)芘、7,12-二甲基苯并(a)蒽、N-甲基-N'-硝基-N-亚硝基胍、硝基喹啉-N-氧化物和铬酸铅）在无外源性代谢激活的情况下诱导形态转化，3种（N-2-芴基乙酰胺、芘和蒽）未产生转化反应。5种编码化学品（2,6-二氯对苯二胺、4,4'-氧二苯胺、肉桂基邻氨基苯甲酸、敌敌畏和利血平）代表环境化学类别，但不一定是强致癌物，在这项实验室间研究中产生了更模糊的反应。因此，虽然该试验在某些情况下可用于区分转化化学品和非转化化学品，但目前该系统在常规检测项目中的使用存在低转化频率和难以获得任何剂量反应结果等固有局限性。为了克服这些局限性，应探索一些增加转化频率或放大转化表型表达的方法。
