Tennant R W, Spalding J W, Stasiewicz S, Caspary W D, Mason J M, Resnick M A
National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709.
Environ Health Perspect. 1987 Nov;75:87-95. doi: 10.1289/ehp.877587.
The results of a recent comprehensive evaluation of the relationship between four measures of in vitro genetic toxicity and the capacity of the chemicals to induce neoplasia in rodents carry some important implications. The results showed that while the Salmonella mutagenesis assay detected only about half of the carcinogens as mutagens, the other three in vitro assays (mutagenesis in MOLY cells or induction of aberrations or SCEs in CHO cells) did not complement Salmonella since they failed to effectively discriminate between the carcinogens and noncarcinogens found negative in the Salmonella assay. The specificity of the Salmonella assay for this group of 73 chemicals was relatively high (only 4 of 29 noncarcinogens were positive). Therefore, we have begun to evaluate in vivo genetic toxicity assays for their ability to complement Salmonella in the identification of carcinogens.
最近对四种体外遗传毒性检测方法与化学物质在啮齿动物中诱发肿瘤能力之间关系进行的全面评估结果具有一些重要意义。结果显示，虽然沙门氏菌诱变试验仅检测到约一半的致癌物为诱变剂，但其他三种体外试验（MOLY细胞中的诱变或CHO细胞中畸变或姐妹染色单体交换的诱导）并不能补充沙门氏菌试验，因为它们未能有效区分在沙门氏菌试验中呈阴性的致癌物和非致癌物。沙门氏菌试验对这73种化学物质的特异性相对较高（29种非致癌物中只有4种呈阳性）。因此，我们已开始评估体内遗传毒性试验在识别致癌物方面补充沙门氏菌试验的能力。
