Sangkhamanon Sakkarn, Kotano Natcha, Sirithanaphol Wichien, Rompsaithong Ukrit, Kiatsopit Pakorn, Sookprasert Aumkhae, Wirasorn Kosin, Twinprai Prin, Watcharenwong Piyakarn, Chindaprasirt Jarin
Department of Pathology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
Division of Urologic Surgery, Department of Surgery, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
Biomed Rep. 2023 May 29;19(1):44. doi: 10.3892/br.2023.1627. eCollection 2023 Jul.
The present study aimed to demonstrate the proportion of the programmed death-ligand 1 (PD-L1) expression in penile cancer patients and the association with clinicopathological parameters. Formalin-fixed paraffin-embedded specimens were obtained from 43 patients with primary penile squamous cell carcinoma treated at Srinagarind Hospital, Faculty of Medicine, Khon Kaen University, between 2008 and 2018. PD-L1 expression was evaluated by the immunohistochemistry using an SP263 monoclonal antibody. PD-L1 positivity was defined as >25% tumor cell staining or >25% tumor-associated immune cell staining. The correlation between PD-L1 expression and clinicopathological parameters was analyzed. A total of eight of 43 patients (18.6%) were identified as positive for PD-L1 expression in tumor cells and tumor-infiltrating lymphocytes. In the PD-L1 positive group, there was a significant association with pathological T stage (P=0.014) with a higher percentage of PD-L1 positive tumors in T1 stage compared with T2-T4 stage. In this cohort, there was a trend towards longer survival in patients with positive PD-L1 expression (5-year OS: 75% vs. 61.2%, P=0.19). Lymph node involvement and the location of tumor at the shaft of penis were two independent prognostic factors for survival. In conclusion, the PD-L1 expression was detected in 18% of penile cancer patients and high expression of PD-L1 was associated with the early T stage.
本研究旨在阐明阴茎癌患者程序性死亡配体1（PD-L1）表达的比例及其与临床病理参数的相关性。从2008年至2018年在孔敬大学医学院诗里拉吉医院接受治疗的43例原发性阴茎鳞状细胞癌患者中获取福尔马林固定石蜡包埋标本。使用SP263单克隆抗体通过免疫组织化学评估PD-L1表达。PD-L1阳性定义为肿瘤细胞染色>25%或肿瘤相关免疫细胞染色>25%。分析PD-L1表达与临床病理参数之间的相关性。43例患者中共有8例（18.6%）被确定为肿瘤细胞和肿瘤浸润淋巴细胞中PD-L1表达阳性。在PD-L1阳性组中，与病理T分期存在显著相关性（P=0.014），与T2-T4期相比，T1期PD-L1阳性肿瘤的百分比更高。在该队列中，PD-L1表达阳性的患者有生存时间更长的趋势（5年总生存率：75%对61.2%，P=0.19）。淋巴结受累和肿瘤位于阴茎体部是生存的两个独立预后因素。总之，在18%的阴茎癌患者中检测到PD-L1表达，且PD-L1高表达与早期T分期相关。
