Tada K, Akamatsu K, Ohta Y
Gastroenterol Jpn. 1986 Jun;21(3):228-37. doi: 10.1007/BF02774565.
The effect of sera from 8 patients with fulminant hepatitis, including 2 survival cases, on DNA and protein synthesis in primary cultured rat hepatocytes was studied. The serum from patients at an early stage or within 10 days after onset tended to intensify DNA synthesis in isolated hepatocytes, whereas the serum from patients with a history of over 50 days distinctly inhibited synthesis. When the serum was fractionated by gel filtration or free-flow electrophoresis, only the albumin fraction inhibited DNA synthesis in cultured hepatocytes. The suppressive effect of the albumin fraction was demonstrated even in patients suffering for only a short period of time. The inhibitory activity against DNA and protein synthesis in cultured hepatocytes was demonstrated in a substance extracted with a chloroform and methanol mixture from the albumin fraction of patients with fulminant hepatitis. The extract from the patients' sera also inhibited acceleration of DNA synthesis by epidermal growth factor (EGF) in the same cells.
研究了8例暴发性肝炎患者（包括2例存活病例）的血清对原代培养大鼠肝细胞DNA和蛋白质合成的影响。发病早期或发病后10天内患者的血清倾向于增强分离肝细胞中的DNA合成，而病程超过50天患者的血清则明显抑制合成。当通过凝胶过滤或自由流动电泳对血清进行分级分离时，只有白蛋白组分抑制培养肝细胞中的DNA合成。即使在患病时间较短的患者中，白蛋白组分也表现出抑制作用。从暴发性肝炎患者白蛋白组分中用氯仿和甲醇混合物提取的一种物质，对培养肝细胞中的DNA和蛋白质合成具有抑制活性。患者血清提取物也抑制同一细胞中表皮生长因子（EGF）对DNA合成的促进作用。
