Laboratory of Developmental Cardiology, Institute of Physiology of the Czech Academy of Sciences, Prague, Czech Republic.
Department of Physiology, Faculty of Science, Charles University, Prague, Czech Republic.
Epigenetics. 2023 Dec;18(1):2218771. doi: 10.1080/15592294.2023.2218771.
Epitranscriptomic modifications have recently emerged into the spotlight of researchers due to their vast regulatory effects on gene expression and thereby cellular physiology and pathophysiology. N,2'-O-dimethyladenosine (mAm) is one of the most prevalent chemical marks on RNA and is dynamically regulated by writers (PCIF1, METTL4) and erasers (FTO). The presence or absence of mAm in RNA affects mRNA stability, regulates transcription, and modulates pre-mRNA splicing. Nevertheless, its functions in the heart are poorly known. This review summarizes the current knowledge and gaps about mAm modification and its regulators in cardiac biology. It also points out technical challenges and lists the currently available techniques to measure mAm. A better understanding of epitranscriptomic modifications is needed to improve our knowledge of the molecular regulations in the heart which may lead to novel cardioprotective strategies.
近年来，由于其对基因表达以及细胞生理和病理生理学的广泛调节作用，转录后修饰已成为研究人员关注的焦点。N ，2'-O-二甲基腺苷（mAm）是 RNA 上最常见的化学标记之一，由写入器（PCIF1、METTL4）和橡皮擦（FTO）动态调节。RNA 中 mAm 的存在或不存在会影响 mRNA 的稳定性、调节转录并调节前体 mRNA 的剪接。然而，其在心脏中的功能知之甚少。本文综述了 mAm 修饰及其在心脏生物学中的调节因子的最新知识和空白，还指出了技术挑战并列出了目前可用于测量 mAm 的技术。需要更好地了解转录后修饰，以提高我们对心脏分子调控的认识，这可能会导致新的心脏保护策略。
