压力超负荷诱导的心肌肥厚中 RNA m6A 甲基化的综合分析。
Comprehensive analysis of RNA m6A methylation in pressure overload-induced cardiac hypertrophy.
机构信息
Department of Physiology and Neurobiology, School of Basic Medical Sciences, Zhengzhou University, No.100 Kexuedadao Road, Zhengzhou, 450000, China.
Department of Pharmacology, School of Basic Medical Sciences, Zhengzhou University, No.100 Kexuedadao Road, Zhengzhou, 450000, China.
出版信息
BMC Genomics. 2022 Aug 11;23(1):576. doi: 10.1186/s12864-022-08833-w.
AIM
To analyze and compare the mRNA N-methyladenosine modifications in transverse aortic constriction induced mice hearts and normal mice hearts.
MATERIALS AND METHODS
Colorimetric quantification was used to probe the changes in mA modifications in the total RNA. The expression of mA-related enzymes was analyzed via qRT-PCR and western blotting. RNA-seq and MeRIP-seq were performed to identify genes with differences in mA modifications or expression in the transcriptome profile.
RESULTS
Compared with the control group, the TAC group exhibited higher mA methylation levels. FTO and WTAP were downregulated after TAC, while METTL3 was significantly downregulated at the protein level. MeRIP-seq revealed that 1179 mA peaks were upmethylated and 733 mA peaks were downmethylated, and biological analysis of these genes exhibited a strong relationship with heart function.
CONCLUSION
Our findings provide novel information regarding mA modification and gene expression changes in cardiac hypertrophy, which may be fundamental for further research.
目的
分析和比较主动脉缩窄诱导的小鼠心脏与正常小鼠心脏中的 mRNA N6-甲基腺苷修饰。
材料与方法
采用比色定量法探测总 RNA 中 mA 修饰的变化。通过 qRT-PCR 和 Western blot 分析 mA 相关酶的表达。进行 RNA-seq 和 MeRIP-seq 以鉴定在转录组图谱中 mA 修饰或表达存在差异的基因。
结果
与对照组相比，TAC 组的 mA 甲基化水平更高。TAC 后 FTO 和 WTAP 下调，而 METTL3 在蛋白水平显著下调。MeRIP-seq 显示 1179 个 mA 峰被高甲基化，733 个 mA 峰被低甲基化，这些基因的生物分析显示与心脏功能有很强的关系。
结论
我们的研究结果为心肌肥厚中 mA 修饰和基因表达变化提供了新的信息，这可能是进一步研究的基础。
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