Li Youzhong, Chen Haihong, Wang Youwu, Zhu Jincheng, Zhang Xiaoli, Sun Jie, Liu Feng, Zhao Yiying
Key Laboratory of Oasis Eco-Agriculture, College of Agriculture, Shihezi University, Shihezi, China.
Xinjiang Production and Construction Group Key Laboratory of Crop Germplasm Enhancement and Gene Resources Utilization, Cotton Research Institute, Xinjiang Academy of Agricultural and Reclamation Science, Shihezi, China.
Front Plant Sci. 2023 Jun 2;14:1203695. doi: 10.3389/fpls.2023.1203695. eCollection 2023.
WRKY transcription factors (TFs) play an important role in regulating the mechanism of plant self-defense. However, the function of most WRKY TFs in upland cotton (Gossypium hirsutum) is still unknown. Hence, studying the molecular mechanism of WRKY TFs in the resistance of cotton to Verticillium dahliae is of great significance to enhancing cotton disease resistance and improving its fiber quality. In this study, Bioinformatics has been used to characterize the cotton WRKY53 gene family. we analyzed the GhWRKY53 expression patterns in different resistant upland cotton cultivars treated with salicylic acid (SA) and methyl jasmonate (MeJA). Additionally, GhWRKY53 was silenced using a virus-induced gene silencing (VIGS) to determine the contribution of GhWRKY53 to V. dahliae resistance in cotton. The result showed that GhWRKY53 mediated SA and MeJA signal transduction pathways. After VIGS of the GhWRKY53, the ability of cotton to resist V. dahliae decreased, indicating that the GhWRKY53 could be involved in the disease resistance mechanism of cotton. Studies on the levels of SA and jasmonic acid (JA) and their related pathway genes demonstrated that the silencing of GhWRKY53 inhibited the SA pathway and activated the JA pathway, thereby reducing the resistance of plants to V. dahliae. In conclusion, GhWRKY53 could change the tolerance of upland cotton to V. dahliae by regulating the expression of SA and JA pathway-related genes. However, the interaction mechanism between JA and SA signaling pathways in cotton in response to V. dahliae requires further study.
WRKY转录因子在调控植物自卫机制中发挥着重要作用。然而，大多数WRKY转录因子在陆地棉（陆地棉）中的功能仍然未知。因此，研究WRKY转录因子在棉花对大丽轮枝菌抗性中的分子机制，对于增强棉花抗病性和改善其纤维品质具有重要意义。在本研究中，利用生物信息学对棉花WRKY53基因家族进行了表征。我们分析了水杨酸（SA）和茉莉酸甲酯（MeJA）处理的不同抗性陆地棉品种中GhWRKY53的表达模式。此外，使用病毒诱导基因沉默（VIGS）使GhWRKY53沉默，以确定GhWRKY53对棉花抗大丽轮枝菌的贡献。结果表明，GhWRKY53介导了SA和MeJA信号转导途径。GhWRKY53进行VIGS后，棉花对大丽轮枝菌的抗性能力下降，表明GhWRKY53可能参与了棉花的抗病机制。对SA和茉莉酸（JA）水平及其相关途径基因的研究表明，GhWRKY53的沉默抑制了SA途径并激活了JA途径，从而降低了植物对大丽轮枝菌的抗性。总之，GhWRKY53可以通过调节SA和JA途径相关基因的表达来改变陆地棉对大丽轮枝菌的耐受性。然而，棉花中JA和SA信号通路在响应大丽轮枝菌时的相互作用机制需要进一步研究。
