Sa-Eed Alhassan, Donkor Eric S, Arhin Reuben E, Tetteh-Quarcoo Patience B, Attah Simon K, Kabotso Daniel E K, Kotey Fleischer C N, Dayie Nicholas T K D
Department of Medical Microbiology, University of Ghana Medical School, P. O. Box KB 4236, Korle Bu, Accra, Ghana.
Department of Medical Laboratory Technology, Accra Technical University, P. O. Box GP 561, Barnes Road, Accra, Ghana.
FEMS Microbes. 2023 Apr 20;4:xtad010. doi: 10.1093/femsmc/xtad010. eCollection 2023.
The search for antimicrobials in propolis presents a new dimension for addressing the problem of antimicrobial drug resistance. The aim of this study was to determine the antimicrobial activity of extracts of crude propolis collected from different regions in Ghana and their active fractions. The antimicrobial activity of the extracts, as well as that of the chloroform, ethyl acetate, and petroleum ether fractions of the active samples were determined using the agar well diffusion method. The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of the most active fractions were determined. The various crude propolis extracts frequently produced zones of inhibition against (17/20) than (16/20), and (1/20) test isolates. Chloroform and ethyl acetate solvents produced fractions possessing greater antimicrobial activity than the petroleum ether fraction. The mean MIC range of the most active fractions was greatest for (76.0 ± 34.8-48.0 ± 33.0 mg/ml) than for (40.8 ± 33.3-30.4 ± 6.7 mg/ml) and , as was the mean MBC. Propolis has antimicrobial potential, and hence should be exploited as an alternative for the treatment of bacterial infections.
在蜂胶中寻找抗菌物质为解决抗菌药物耐药性问题提供了一个新的方向。本研究的目的是测定从加纳不同地区采集的粗蜂胶提取物及其活性成分的抗菌活性。采用琼脂孔扩散法测定提取物以及活性样品的氯仿、乙酸乙酯和石油醚馏分的抗菌活性。测定了最具活性馏分的最低抑菌浓度（MIC）和最低杀菌浓度（MBC）。各种粗蜂胶提取物对测试菌株产生抑菌圈的频率通常为（17/20）高于（16/20）和（1/20）。氯仿和乙酸乙酯溶剂产生的馏分比石油醚馏分具有更强的抗菌活性。最具活性馏分的平均MIC范围对（76.0±34.8 - 48.0±33.0毫克/毫升）大于对（40.8±33.3 - 30.4±6.7毫克/毫升）和，平均MBC也是如此。蜂胶具有抗菌潜力，因此应作为治疗细菌感染的替代品加以利用。
