Department of Microbiology, Centre for Scientific Research into Plant Medicine, Mampong-Akwapim, Ghana.
Ann Clin Microbiol Antimicrob. 2012 Jun 18;11:16. doi: 10.1186/1476-0711-11-16.
Following claims that some plants have antimicrobial activities against infectious microbes, the in vitro antimicrobial activities of different solvent fractions of ethanolic extract of Cryptolepis sanguinolenta were evaluated against eight standard bacteria and clinical isolates.
The solvent partitioning protocol involving ethanol, petroleum ether, chloroform, ethyl acetate and water, was used to extract various fractions of dried pulverized Cryptolepis sanguinolenta roots. Qualitative phyto-constituents screening was performed on the ethanol extract, chloroform fraction and the water fraction. The Kirby Bauer disk diffusion method was employed to ascertain the antibiogram of the test organisms while the agar diffusion method was used to investigate the antimicrobial properties of the crude plant extracts. The microplate dilution method aided in finding the MICs while the MBCs were obtained by the method of Nester and friends. The SPSS 16.0 version was used to analyze the percentages of inhibitions and bactericidal activities.
The phytochemical screening revealed the presence of alkaloids, reducing sugars, polyuronides, anthocyanosides and triterpenes. The ethanol extract inhibited 5 out of 8 (62.5%) of the standard organisms and 6 out of 8 (75%) clinical isolates. The petroleum ether fraction inhibited 4 out of 8 (50%) of the standard microbes and 1 out of 8 (12.5%) clinical isolates. It was also observed that the chloroform fraction inhibited the growth of all the organisms (100%). Average inhibition zones of 14.0 ± 1.0 mm to 24.67 ± 0.58 mm was seen in the ethyl acetate fraction which halted the growth of 3 (37.5%) of the standard organisms. Inhibition of 7 (87.5%) of standard strains and 6 (75%) of clinical isolates were observed in the water fraction. The chloroform fraction exhibited bactericidal activity against all the test organisms while the remaining fractions showed varying degrees of bacteriostatic activity.
The study confirmed that fractions of Cryptolepis sanguinolenta have antimicrobial activity. The chloroform fraction had the highest activity, followed by water, ethanol, petroleum ether and ethyl acetate respectively. Only the chloroform fraction exhibited bactericidal activity and further investigations are needed to ascertain its safety and prospects of drug development.
有报道称一些植物对感染性微生物具有抗菌活性，因此评估了乙醇提取物的不同溶剂部分对八种标准细菌和临床分离株的体外抗菌活性。
使用乙醇、石油醚、氯仿、乙酸乙酯和水的溶剂分配方案提取干燥粉碎的Cryptolepis sanguinolenta 根的各种部分。对乙醇提取物、氯仿部分和水部分进行定性植物成分筛选。采用 Kirby Bauer 圆盘扩散法确定测试菌的抗生素谱，采用琼脂扩散法研究粗提物的抗菌特性。微量稀释法有助于确定 MIC，而 Nester 等人的方法则用于确定 MBC。使用 SPSS 16.0 版本分析抑制率和杀菌活性的百分比。
植物化学筛选显示存在生物碱、还原糖、多聚糖、花色苷和三萜类。乙醇提取物抑制了 8 种标准菌中的 5 种（62.5%）和 8 种临床分离株中的 6 种（75%）。石油醚部分抑制了 8 种标准微生物中的 4 种（50%）和 8 种临床分离株中的 1 种（12.5%）。还观察到氯仿部分抑制了所有微生物的生长（100%）。乙酸乙酯部分的平均抑制带为 14.0±1.0mm 至 24.67±0.58mm，可阻止 3 种（37.5%）标准菌的生长。水部分抑制了 7 种（87.5%）标准株和 6 种（75%）临床分离株的生长。氯仿部分对所有测试菌均具有杀菌活性，而其余部分则表现出不同程度的抑菌活性。
该研究证实，Cryptolepis sanguinolenta 的部分具有抗菌活性。氯仿部分活性最高，其次是水、乙醇、石油醚和乙酸乙酯。只有氯仿部分表现出杀菌活性，需要进一步研究以确定其安全性和药物开发前景。
