Kakagianni Myrsini, Tsiknia Myrto, Feka Maria, Vasileiadis Sotirios, Leontidou Kleopatra, Kavroulakis Nektarios, Karamanoli Katerina, Karpouzas Dimitrios G, Ehaliotis Constantinos, Papadopoulou Kalliope K
Department of Biochemistry and Biotechnology, Laboratory of Plant and Environmental Biotechnology, University of Thessaly, Larissa 41500, Greece.
Department of Food Science and Nutrition, School of Agricultural Sciences, University of Thessaly, Temponera str, 43100 Karditsa, Greece.
FEMS Microbes. 2023 Jan 6;4:xtad001. doi: 10.1093/femsmc/xtad001. eCollection 2023.
The olive tree is a hallmark crop in the Mediterranean region. Its cultivation is characterized by an enormous variability in existing genotypes and geographical areas. As regards the associated microbial communities of the olive tree, despite progress, we still lack comprehensive knowledge in the description of these key determinants of plant health and productivity. Here, we determined the prokaryotic, fungal and arbuscular mycorrhizal fungal (AMF) microbiome in below- (rhizospheric soil, roots) and above-ground (phyllosphere and carposphere) plant compartments of two olive varieties 'Koroneiki' and 'Chondrolia Chalkidikis' grown in Southern and Northern Greece respectively, in five developmental stages along a full fruit-bearing season. Distinct microbial communities were supported in above- and below-ground plant parts; while the former tended to be similar between the two varieties/locations, the latter were location specific. In both varieties/locations, a seasonally stable root microbiome was observed over time; in contrast the plant microbiome in the other compartments were prone to changes over time, which may be related to seasonal environmental change and/or to plant developmental stage. We noted that olive roots exhibited an AMF-specific filtering effect (not observed for bacteria and general fungi) onto the rhizosphere AMF communities of the two olive varieties/locations/, leading to the assemblage of homogenous intraradical AMF communities. Finally, shared microbiome members between the two olive varieties/locations include bacterial and fungal taxa with putative functional attributes that may contribute to olive tree tolerance to abiotic and biotic stress.
橄榄树是地中海地区的标志性作物。其种植的特点是现有基因型和地理区域存在巨大差异。关于橄榄树相关的微生物群落，尽管取得了进展，但我们在描述这些植物健康和生产力的关键决定因素方面仍缺乏全面的知识。在这里，我们测定了分别生长在希腊南部和北部的两个橄榄品种“科罗内基”和“软骨藻基基迪斯”在整个结果季节的五个发育阶段中，地下（根际土壤、根系）和地上（叶际和果实表面）植物部分的原核生物、真菌和丛枝菌根真菌（AMF）微生物组。地上和地下植物部分支持不同的微生物群落；虽然前者在两个品种/地点之间趋于相似，但后者具有地点特异性。在两个品种/地点，随着时间的推移都观察到了季节性稳定的根系微生物组；相比之下，其他部分的植物微生物组容易随时间发生变化，这可能与季节性环境变化和/或植物发育阶段有关。我们注意到，橄榄树根对两个橄榄品种/地点的根际AMF群落表现出AMF特异性的过滤作用（细菌和普通真菌未观察到），导致形成同质的根内AMF群落。最后，两个橄榄品种/地点之间共有的微生物组成员包括具有推定功能属性的细菌和真菌分类群，这些分类群可能有助于橄榄树耐受非生物和生物胁迫。
