Wang Jie, Peng Liang, Han Dongxue, Zheng Teng, Chang Tianying, Cui Hong-Liang
School of Information and Electrical Engineering, Hangzhou City University, Hangzhou, China.
Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China.
Front Microbiol. 2023 Jun 2;14:1195448. doi: 10.3389/fmicb.2023.1195448. eCollection 2023.
In recent years, terahertz (THz) imaging has attracted much attention because of its ability to obtain physical and chemical information in a label-free, noninvasive and nonionizing manner. However, the low spatial resolution of traditional THz imaging systems and the weak dielectric response of biological samples hinder the application of this technology in the biomedical field. In this paper, we report a new THz near-field imaging method for a single bacteria, through the coupling effect of nanoscale radius of probe and platinum gold substrate, which greatly enhances THz near-field signal of biological samples. A THz super-resolution image of bacteria has been successfully obtained by strictly controlling the relevant test parameters such as tip parameters and driving amplitude. By analyzing and processing the THz spectral image, the morphology and inner structure of bacteria have been observed. The method has been used to detect and identify represented by Gram-negative bacteria and represented by Gram-positive bacteria. This application provides a new label-free, noninvasive and nonionizing testing protocol for the detection of single bacteria.
近年来，太赫兹（THz）成像因其能够以无标记、非侵入性和非电离的方式获取物理和化学信息而备受关注。然而，传统太赫兹成像系统的低空间分辨率以及生物样品的弱介电响应阻碍了该技术在生物医学领域的应用。在本文中，我们报告了一种针对单个细菌的新型太赫兹近场成像方法，通过探针纳米级半径与铂金基底的耦合效应，极大地增强了生物样品的太赫兹近场信号。通过严格控制诸如针尖参数和驱动幅度等相关测试参数，成功获得了细菌的太赫兹超分辨率图像。通过对太赫兹光谱图像进行分析和处理，观察到了细菌的形态和内部结构。该方法已用于检测和识别以革兰氏阴性菌为代表的[具体细菌种类1]和以革兰氏阳性菌为代表的[具体细菌种类2]。此应用为单个细菌的检测提供了一种新的无标记、非侵入性和非电离测试方案。
