Chen Andrew, Virk Arjun, Harris Zachery, Abazari Azin, Honkanen Robert, Arbab M Hassan
Department of Biomedical Engineering, Stony Brook University, 100 Nicolls Rd, Stony Brook, NY 11794, USA.
Department of Ophthalmology, Renaissance School of Medicine, 101 Nicolls Rd, Stony Brook, NY 11794, USA.
Biomed Opt Express. 2021 May 17;12(6):3438-3449. doi: 10.1364/BOE.423741. eCollection 2021 Jun 1.
Elevated intraocular pressure (IOP) results in endothelial layer damage that can induce corneal hydration perturbations. We investigated the potential of terahertz spectroscopy in measuring the IOP levels through mapping corneal water content. We controlled the IOP levels in rabbit and porcine eye samples while monitoring the change in corneal hydration using a terahertz time-domain spectroscopy (THz-TDS) scanner. Our results showed a statistically significant increase in the THz reflectivity between 0.4 and 0.6 THz corresponding to the increase in the IOP. Endothelial layer damage was confirmed using scanning electron microscopy (SEM) of the corneal biopsy samples. Our empirical results indicate that the THz-TDS can be used to track IOP levels through the changes in corneal hydration.
眼内压（IOP）升高会导致内皮细胞层损伤，进而引发角膜水化紊乱。我们研究了太赫兹光谱技术通过绘制角膜含水量来测量眼内压水平的潜力。我们在控制兔眼和猪眼样本眼内压水平的同时，使用太赫兹时域光谱（THz-TDS）扫描仪监测角膜水化的变化。我们的结果显示，在0.4至0.6太赫兹之间，太赫兹反射率随着眼内压的升高而出现具有统计学意义的增加。通过对角膜活检样本进行扫描电子显微镜（SEM）检查，证实了内皮细胞层损伤。我们的实验结果表明，太赫兹时域光谱技术可通过角膜水化的变化来追踪眼内压水平。
