Shridharani Sachin M, Vijayan Saji, Napekoski Karl M, Dalton Shannon R, Bhatia Ashish C
Aesthet Surg J Open Forum. 2023 Apr 25;5:ojad034. doi: 10.1093/asjof/ojad034. eCollection 2023.
Cellulite is a common aesthetic condition that affects predominantly females. Collagenase clostridium histolyticum-aaes (CCH-aaes) injections disrupt native collagen structures, resulting in an improvement in cellulite appearance. However, injection-site bruising is a frequently occurring adverse event with CCH-aaes treatment.
To characterize tissue histology following CCH-aaes injection in Yorkshire pigs.
In an animal study, female swine with 10 defined dosing sites on the ventral-lateral aspect received 1 or 2 CCH-aaes (0.07 mg/0.3 mL) or placebo subcutaneous injections at a single site at designated time points before tissue sampling.
Injection with CCH-aaes was associated with lysis of mature, collagen-rich septa in the subcutaneous layer at and adjacent to the injection site as early as Day 1. On Day 4, an increase in inflammatory cells and a decrease in hemorrhage (vs Day 2) were observed, with inflammation and hemorrhage decreased by Day 8. By Day 21, deposition of new collagen and reorganization of fat lobules were observed. Observations with repeat CCH-aaes treatment were comparable with 1 course of CCH-aaes treatment.
In this animal study, targeted enzymatic subcision of collagenous bands and remodeling of subcutaneous tissue were observed following CCH-aaes injection.
橘皮组织是一种常见的美容问题，主要影响女性。注射溶组织梭菌胶原酶-aaes（CCH-aaes）会破坏天然胶原蛋白结构，从而改善橘皮组织外观。然而，注射部位瘀伤是CCH-aaes治疗中经常出现的不良事件。
描述约克郡猪注射CCH-aaes后的组织组织学特征。
在一项动物研究中，在腹外侧有10个明确给药部位的雌性猪在组织采样前的指定时间点于单个部位接受1次或2次CCH-aaes（0.07mg/0.3mL）或安慰剂皮下注射。
早在第1天，注射CCH-aaes就与注射部位及附近皮下层中成熟的、富含胶原蛋白的间隔溶解有关。在第4天，观察到炎症细胞增加，出血减少（与第2天相比），到第8天炎症和出血减轻。到第21天，观察到新胶原蛋白的沉积和脂肪小叶的重新组织。重复CCH-aaes治疗的观察结果与1个疗程的CCH-aaes治疗相当。
在这项动物研究中，注射CCH-aaes后观察到胶原带的靶向酶切和皮下组织重塑。
