Liu Haijiang, Pan Yuan, Cui Rui, Hammond John P, White Philip J, Zhang Yuting, Zou Maoyan, Ding Guangda, Wang Sheliang, Cai Hongmei, Xu Fangsen, Shi Lei
National Key Lab of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070 China.
Key Lab of Cultivated Land Conservation, Ministry of Agriculture and Rural Affairs/Microelement Research Centre, Huazhong Agricultural University, Wuhan, 430070 China.
Mol Breed. 2023 Jun 16;43(7):53. doi: 10.1007/s11032-023-01399-9. eCollection 2023 Jul.
Oilseed rape ( L.; ) is an important oil crop worldwide. However, the genetic mechanisms of adaptations to low phosphate (P) stress are largely unknown. In this study, a genome-wide association study (GWAS) identified 68 SNPs significantly associated with seed yield (SY) under low P (LP) availability, and 7 SNPs significantly associated with phosphorus efficiency coefficient (PEC) in two trials. Among these SNPs, two, chrC07__39807169 and chrC09__14194798, were co-detected in two trials, and and were identified as candidate genes of them, respectively, by combining GWAS with quantitative reverse-transcription PCR (qRT-PCR). There were significant differences in the gene expression level of and between P-efficient and -inefficiency varieties at LP. SY_LP had a significant positive correlation with the gene expression level of both and . and could directly bind the promoters of and , respectively. Selective sweep analysis was conducted between ancient and derived , and detected 1280 putative selective signals. Within the selected region, a large number of genes related to P uptake, transport, and utilization were detected, such as purple acid phosphatase (PAP) family genes and phosphate transporter (PHT) family genes. These findings provide novel insights into the molecular targets for breeding P efficiency varieties in .
The online version contains supplementary material available at 10.1007/s11032-023-01399-9.
油菜（L.；）是全球重要的油料作物。然而，其适应低磷（P）胁迫的遗传机制在很大程度上尚不清楚。在本研究中，全基因组关联研究（GWAS）在两项试验中鉴定出68个与低磷（LP）条件下种子产量（SY）显著相关的单核苷酸多态性（SNP），以及7个与磷效率系数（PEC）显著相关的SNP。在这些SNP中，两个，即chrC07__39807169和chrC09__14194798，在两项试验中被共同检测到，并且通过将GWAS与定量逆转录PCR（qRT-PCR）相结合，分别将 和 鉴定为它们的候选基因。在LP条件下，磷高效和低效品种之间的 和 的基因表达水平存在显著差异。SY_LP与 和 的基因表达水平均呈显著正相关。 和 可分别直接结合 和 的启动子。对古老和衍生的 进行了选择性扫描分析，检测到1280个推定的选择信号。在选定区域内，检测到大量与磷吸收、运输和利用相关的基因，如紫色酸性磷酸酶（PAP）家族基因和磷酸盐转运体（PHT）家族基因。这些发现为油菜磷效率品种育种的分子靶点提供了新的见解。
在线版本包含可在10.1007/s11032-023-01399-9获取的补充材料。
