Liu Haijiang, Zou Maoyan, Zhang Bingbing, Yang Xinyu, Yuan Pan, Ding Guangda, Xu Fangsen, Shi Lei
National Key Lab of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070 Hubei Province China.
Key Lab of Cultivated Land Conservation, Ministry of Agriculture and Rural Affairs/Microelement Research Centre, Huazhong Agricultural University, Wuhan, 430070 Hubei Province China.
Mol Breed. 2022 Sep 27;42(10):61. doi: 10.1007/s11032-022-01332-6. eCollection 2022 Oct.
Oilseed rape ( L.) is one of the most essential oil crops. Genetic improvement of seed yield (SY) is a major aim of breeding. Several studies have been reported on the genetic mechanisms of SY of Here, a genome-wide association study (GWAS) of SY was conducted using a panel of 403 natural accessions of , with more than five million high-quality single-nucleotide polymorphisms (SNPs). A total of 1773 significant SNPs were detected associated with SY, and 783 significant SNPs were co-located with previously reported QTLs. The lead SNPs chrA01__8920351 and chrA02__4555979 were jointly detected in Trial 2_2 and Trial 2_mean value, and in Trial 1_2 and Trial 1_mean value, respectively. Subsequently, two candidate genes of and were identified through combining transcriptome, candidate gene association analysis, and haplotype analysis. detected through lead SNP chrA09__5160639 was associated with SY of . Our results provide valuable information for studying the genetic control of seed yield in and valuable genes, haplotypes, and cultivars resources for the breeding of high seed yield cultivars.
The online version contains supplementary material available at 10.1007/s11032-022-01332-6.
油菜(L.)是最重要的油料作物之一。种子产量(SY)的遗传改良是育种的主要目标。关于油菜种子产量的遗传机制已有多项研究报道。在此,利用一组403份油菜自然种质材料进行了种子产量的全基因组关联研究(GWAS),这些材料具有超过500万个高质量单核苷酸多态性(SNP)。共检测到1773个与种子产量相关的显著SNP,其中783个显著SNP与先前报道的数量性状位点(QTL)共定位。主效SNP chrA01__8920351和chrA02__4555979分别在试验2_2和试验2平均值中,以及试验1_2和试验1平均值中被联合检测到。随后,通过整合转录组、候选基因关联分析和单倍型分析,鉴定出两个候选基因。通过主效SNP chrA09__5160639检测到的与油菜种子产量相关。我们的结果为研究油菜种子产量的遗传控制提供了有价值的信息,并为高种子产量品种的育种提供了有价值的基因、单倍型和品种资源。
在线版本包含可在10.1007/s11032-022-01332-6获取的补充材料。