Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China.
College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China.
Theor Appl Genet. 2021 May;134(5):1329-1341. doi: 10.1007/s00122-021-03774-6. Epub 2021 Jan 28.
We detected a QTL qHSW-16 undergone strong selection associated with seed weight and identified a novel candidate gene controlling seed weight candidate gene for this major QTL by qRT-PCT. Soybean [Glycine max (L.) Merr.] provides more than half of the world's oilseed production. To expand its germplasm resources useful for breeding increased yield and oil quality cultivars, it is necessary to resolve the diversity and evolutionary history of this crop. In this work, we resequenced 283 soybean accessions from China and obtained a large number of high-quality SNPs for investigation of the population genetics that underpin variation in seed weight and other agronomic traits. Selective signature analysis detected 78 (~ 25.0 Mb) and 39 (~ 22.60 Mb) novel putative selective signals that were selected during soybean domestication and improvement, respectively. Genome-wide association study (GWAS) identified five loci associated with seed weight. Among these QTLs, qHSW-16, overlapped with the improvement-selective region on chromosome 16, suggesting that this QTL may be underwent strong selection during soybean improvement. Of the 18 candidate genes in qHSW-16, only SoyZH13_16G122400 showed higher expression levels in a large seed variety compared to a small seed variety during seed development. These results identify SoyZH13_16G122400 as a novel candidate gene controlling seed weight and provide foundational insights into the molecular targets for breeding improvement of seed weight and potential seed yield in soybean.
我们检测到一个与种子重量强烈相关的 QTL qHSW-16，并通过 qRT-PCT 鉴定了一个控制这个主要 QTL 种子重量的新候选基因。大豆[Glycine max (L.) Merr.]提供了世界上一半以上的油籽产量。为了扩大其用于培育增加产量和改善油质品种的种质资源，有必要解决这种作物的多样性和进化历史。在这项工作中，我们对来自中国的 283 个大豆品种进行了重测序，获得了大量高质量的 SNP，用于研究支持种子重量和其他农艺性状变异的群体遗传学。选择信号分析检测到 78（25.0 Mb）和 39（22.60 Mb）个新的假定选择信号，分别在大豆驯化和改良过程中受到选择。全基因组关联研究（GWAS）鉴定了与种子重量相关的五个位点。在这些 QTL 中，qHSW-16 与第 16 号染色体上的改良选择区域重叠，表明该 QTL 可能在大豆改良过程中受到强烈选择。在 qHSW-16 的 18 个候选基因中，只有 SoyZH13_16G122400 在种子发育过程中在大粒品种中的表达水平高于小粒品种。这些结果将 SoyZH13_16G122400 鉴定为控制种子重量的新候选基因，并为大豆种子重量和潜在种子产量的育种改良提供了分子靶标方面的基础见解。
