Key Laboratory of Industrial Biocatalysis, Ministry of Education, Department of Chemical Engineering, Tsinghua University, Tsinghua-Peking Center of Life Sciences, Beijing 100084, China.
University of Chinese Academy of Sciences, Beijing, 100049, China.
J Mater Chem B. 2022 Aug 17;10(32):6214-6227. doi: 10.1039/d2tb01122k.
The hierarchical three-dimensional (3D)-printing scaffolds based on microbial polyester poly(3-hydroxybutyrate--4-hydroxybutyrate) (P34HB) were designed and used for bone tissue engineering surface functionalization on 3D-printed (P34HB) scaffolds using polydopamine (PDA)-mediated recombinant human bone morphogenetic protein-2 (BMP2), leading to enhanced bone formation in a rat model with a calvarial critical-size bone defect. Taking advantage of the adhesive property of PDA under alkaline and aerobic conditions, osteogenic BMP2 was captured on the surface of PHA scaffolds, resulting in their enhanced osteogenic bioactivity, better stem cell adhesion and proliferation, and sustainable release of a bioactive substance over a period of 30 days. These contributed to notable differences in alkaline phosphatase (ALP) activity, mineralization, expressions of osteogenesis-related genes, as well as finally enhanced bone formation in rats. The functionalized 3D-printed P34HB scaffolds the PDA-mediated osteogenic activity were developed as a versatile platform for bone tissue regeneration.
基于微生物聚酯聚(3-羟基丁酸酯-4-羟基丁酸酯) (P34HB) 的分层三维(3D)打印支架被设计并用于骨组织工程，通过聚多巴胺(PDA)介导的重组人骨形态发生蛋白-2 (BMP2)对 3D 打印(P34HB)支架进行表面功能化，导致大鼠颅骨临界大小骨缺损模型中的骨形成增强。利用 PDA 在碱性和有氧条件下的粘附特性，成骨 BMP2 被捕获在 PHA 支架的表面，从而提高了它们的成骨生物活性，更好地促进了干细胞的黏附和增殖，并在 30 天的时间内持续释放生物活性物质。这导致碱性磷酸酶(ALP)活性、矿化、成骨相关基因表达以及最终在大鼠中增强骨形成方面有显著差异。功能化的 3D 打印 P34HB 支架和 PDA 介导的成骨活性被开发为一种用于骨组织再生的多功能平台。
