Li Mengjie, Chen Mengmeng, Yang Facui, Qin Rongrong, Yang Qingmin, Ren Hao, Liu Han, Yang Peng
Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an, 710119, China.
Xi'an Key Laboratory of Polymeric Soft Matter, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an, 710119, China.
Adv Healthc Mater. 2023 Oct;12(26):e2300999. doi: 10.1002/adhm.202300999. Epub 2023 Jul 12.
Biocompatible adsorbents play an essential role in hemoperfusion. Nevertheless, there are no hemoperfusion adsorbents that can simultaneously remove small and medium toxins, including bilirubin, urea, phosphor, heavy metals, and antibiotics. This bottleneck significantly impedes the miniaturization and portability of hemoperfusion materials and devices. Herein, a biocompatible protein-polysaccharide complex is reported that exhibits "multi-in-one" removal efficacy for liver and kidney metabolism wastes, toxic metal ions, and antibiotics. Through electrostatic interactions and polysaccharide-mediated coacervation, adsorbents can be prepared by simply mixing lysozyme (LZ) and sodium alginate (SA) together in seconds. The LZ/SA absorbent presented high adsorption capacities for bilirubin, urea, and Hg of up to 468, 331, and 497 mg g , respectively, and the excellent anti-protein adsorption endowed LZ/SA with a record-high adsorption capacity for bilirubin in the interference of serum albumin to simulate the physiological environment. The LZ/SA adsorbent also has effective adsorption capacity for heavy metals (Pb , Cu , Cr , and Cd ) and multiple antibiotics (terramycin, tetracycline, enrofloxacin, norfloxacin, roxithromycin, erythromycin, sulfapyrimidine, and sulfamethoxazole). Various adsorption functional groups exposed on the adsorbent surface significantly contribute to the excellent adsorption capacity. This fully bio-derived protein/alginate-based hemoperfusion adsorbent has great application prospects in the treatment of blood-related diseases.
生物相容性吸附剂在血液灌流中起着至关重要的作用。然而，目前尚无能够同时去除中小分子毒素的血液灌流吸附剂，这些毒素包括胆红素、尿素、磷、重金属和抗生素等。这一瓶颈严重阻碍了血液灌流材料和装置的小型化与便携性。在此，我们报道了一种生物相容性蛋白质 - 多糖复合物，它对肝肾代谢废物、有毒金属离子和抗生素具有“多合一”的去除效果。通过静电相互作用和多糖介导的凝聚作用，只需将溶菌酶（LZ）和海藻酸钠（SA）在数秒内简单混合，即可制备出吸附剂。LZ/SA吸附剂对胆红素、尿素和汞的吸附容量分别高达468、331和497 mg g，并且其优异的抗蛋白质吸附性能使LZ/SA在血清白蛋白干扰下模拟生理环境时对胆红素具有创纪录的高吸附容量。LZ/SA吸附剂对重金属（Pb、Cu、Cr和Cd）和多种抗生素（土霉素、四环素、恩诺沙星、诺氟沙星、罗红霉素、红霉素、磺胺嘧啶和磺胺甲恶唑）也具有有效的吸附能力。吸附剂表面暴露的各种吸附官能团显著促成了其优异的吸附容量。这种完全生物衍生的基于蛋白质/藻酸盐的血液灌流吸附剂在血液相关疾病的治疗中具有广阔的应用前景。
