Laboratori de Botànica, Facultat de Farmàcia i Ciències de l'Alimentació; Institut de Recerca de la Biodiversitat (IRBio) & Centre de Documentació de Biodiversitat Vegetal (CeDocBiV), Universitat de Barcelona, Barcelona, Spain.
Instituto de Ciencias Biomédicas, Universidad Autónoma de Chile, Temuco, Chile.
Methods Mol Biol. 2023;2672:65-77. doi: 10.1007/978-1-0716-3226-0_3.
Fluorimetry analysis of nuclear DNA content allows identification of genome size and ploidy levels of different life phases, tissues, and populations in seaweed species. It is an easy method that saves time and resources compared to more complex techniques. Here we describe the methodology for measuring nuclear DNA content in seaweed species by DAPI fluorochrome staining and its comparison with the standard Gallus gallus erythrocytes nuclear content, one of the preferred internal standards. With this methodology, up to a thousand nuclei can be measured in a single staining session, allowing for a quick analysis of the studied species.
荧光光度分析核 DNA 含量可用于鉴定海藻物种不同生活阶段、组织和种群的基因组大小和倍性水平。与更复杂的技术相比，该方法省时省力。本文描述了通过 DAPI 荧光染料染色测量海藻核 DNA 含量的方法，并将其与首选内参标准之一的鸡红细胞核标准进行了比较。采用该方法，在单次染色中可测量多达 1000 个核，可快速分析研究物种。
