This study aimed to investigate the application value of an interactive micro class on occupational protection in the operating room for nursing interns. Using a cluster sampling approach, 200 junior college nursing interns were selected as participants at our hospital, who were practicing from June 2020 to April 2021. They were randomly assigned to either the observation group or the control group, with 100 participants in each. Data on teaching-related evaluation indicators, including clarity of teaching objectives, learning atmosphere, rational use of learning resources, effectiveness of teaching process regulation, and degree of students' participation in activities, were collected for both groups. Additionally, the scores of occupational protection assessment in the operating room, which encompassed physical, chemical, biological, environmental, and physiological and psychological factors, were also recorded. The comparative analysis of teaching-related evaluation indicators between the 2 groups revealed statistically significant differences. Specifically, significant differences were found between the 2 groups in the clarity of teaching objectives (P = .007), learning atmosphere (P < .001), rational use of learning resources (P < .001), effectiveness of teaching process regulation (P = .01), and degree of students' participation in activities (P < .001). Furthermore, the scores of all items in the observation group were higher than those in the control group. Additionally, the comparison results of the scores of the occupational protection assessment in the operating room between the 2 groups showed that while there was no statistically significant difference between the 2 groups before the intervention (P > .05). However, after the intervention, statistically significant differences were found between the 2 groups in physical (P < .001), chemical (P = .001), biological (P < .001), environmental (P < .001), and physiological and psychological factors (P < .001). Additionally, the scores of all items in the observation group were higher than those in the control group. The implementation of the interactive micro class enhanced the quality of teaching related to occupational protection in the operating room for interning nurses, thus verifying its benefits in clinical teaching practices.