The Second Affiliated Hospital of Shandong First Medical University, Tai'an, China.
Children's Hospital Affiliated to Suzhou University, Suzhou, China.
Medicine (Baltimore). 2023 May 19;102(20):e33730. doi: 10.1097/MD.0000000000033730.
Febrile seizure (FS) is a highly recurrent neuro-system disorder in children that affects their nervous system development and quality of life. However, the pathogenesis of febrile seizures remains unclear. Our study aims to investigate the potential differences in the intestinal flora and metabolomics between healthy children and those with FS. By examining the relationship between specific flora and different metabolites, we hope to shed light on the pathogenesis of FS. Fecal specimens were collected from healthy children (n = 15) and children with febrile seizures (n = 15), and 16S rDNA sequencing was conducted to characterize intestinal flora. Subsequently, fecal samples from healthy (n = 6) and febrile seizure children (n = 6) were used to characterize metabolomics using linear discriminant analysis of effect size, orthogonal partial least squares discriminant analysis, Kyoto Encyclopedia of Genes and Genomes (pathway enrichment analysis), and Kyoto encyclopedia of genes and genomes topology analysis. Liquid chromatography-mass spectrometry was used to identify metabolites in the fecal samples. The intestinal microbiome in the febrile seizure children significantly differed from that in the healthy children at the phylum level. Ten differentially accumulated metabolites (xanthosine, (S)-abscisic acid, N-palmitoylglycine, (+/-)-2-(5-methyl-5-vinyl-tetrahydrofuran-2-yl) propionaldehyde, (R)-3-hydroxybutyrylcarnitine, lauroylcarnitine, oleoylethanolamide, tetradecyl carnitine, taurine, and lysoPC [18:1 (9z)/0:0] were considered the potential febrile seizure markers. Three metabolic pathways (taurine metabolism; glycine, serine, and threonine metabolism; and arginine biosynthesis) were found essential in febrile seizure. Bacteroides were significantly correlated with the 4 differential metabolites. Adjusting the balance of intestinal flora may be an effective method for preventing and treating febrile seizures.
热性惊厥（FS）是一种高度复发性的儿童神经系统疾病，会影响其神经系统发育和生活质量。然而，热性惊厥的发病机制仍不清楚。我们的研究旨在探讨健康儿童和 FS 儿童之间肠道菌群和代谢组学的潜在差异。通过检查特定菌群与不同代谢物之间的关系，我们希望能深入了解 FS 的发病机制。收集健康儿童（n=15）和热性惊厥儿童（n=15）的粪便标本，进行 16S rDNA 测序以表征肠道菌群。随后，使用线性判别分析效应大小、正交偏最小二乘判别分析、京都基因与基因组百科全书（途径富集分析）和京都基因与基因组拓扑分析，对健康（n=6）和热性惊厥儿童（n=6）的粪便样本进行代谢组学分析。使用液相色谱-质谱法鉴定粪便样本中的代谢物。FS 儿童的肠道微生物组在门水平上与健康儿童显著不同。有 10 种差异积累的代谢物（黄嘌呤核苷、（S）-脱落酸、N-棕榈酰甘氨酸、（±）-2-（5-甲基-5-乙烯基四氢呋喃-2-基）丙醛、（R）-3-羟基丁酰肉碱、月桂酰肉碱、油酰乙醇酰胺、十四烷酰肉碱、牛磺酸和溶血磷脂酰胆碱[18：1（9z）/0：0]被认为是潜在的热性惊厥标志物。发现三个代谢途径（牛磺酸代谢；甘氨酸、丝氨酸和苏氨酸代谢；精氨酸生物合成）在热性惊厥中至关重要。拟杆菌与 4 种差异代谢物显著相关。调节肠道菌群的平衡可能是预防和治疗热性惊厥的有效方法。
